Product Name	ZNF420
Chinese Name	Zinc finger protein420抗体
Alias	APAK; ATM and p53 associated KZNF protein; FLJ32191; Zinc finger protein 420; ZNF 420; ZN420_HUMAN.
Research Area	Cell biology  Apoptosis  Cyclin  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Pig, Cow, Horse, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	80kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ZNF420: 301-688/688
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	ZNF420 contains 19 C2H2-type zinc fingers and 1 KRAB domain. It may be involved in transcriptional regulation. It is a negative regulator of p53-mediated apoptosis. Function: May be involved in transcriptional regulation. Subcellular Location: Nucleus (Potential). Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 19 C2H2-type zinc fingers. Contains 1 KRAB domain. SWISS: Q8TAQ5 Gene ID: 147923 Database links: UniProtKB/Swiss-Prot: Q8TAQ5.1
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF420) Polyclonal Antibody, Unconjugated (SL7092R) at 1:5000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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