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Product Name ERR-alpha Chinese Name 雌激素受体相关蛋白α抗体 Alias Estrogen Related Receptor alpha; ERR-α; ERR α; ERRα; ERR alpha; Err 1; ERR a; ERR alpha; ERR-alpha; Err1; ERR1_HUMAN; ERRa; ERRalpha; ESRL 1; ESRL1; ESRR A; Esrra; Estrogen receptor like 1; Estrogen receptor related 1; Estrogen receptor-like 1; Estrogen related receptor alpha; Estrogen-related receptor alpha; Estrra; Nr3b1; Nuclear receptor subfamily 3 group B member 1; Steroid hormone receptor ERR1. literatures Specific References (2) | SL6998R has been referenced in 2 publications.[IF=3.159] Wan Boyang. et al. Zearalenone promotes follicle development through activating SIRT1/PGC-1α signaling pathway in the ovaries of weaned gilts. J Anim Sci. 2022 Feb;: WB ; Pig(Gilt).[IF=2.447] Panpan Dong. et al. Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells. Exp Ther Med. 2022 Feb;23(2):1-10 WB ; Human.Research Area Tumour Cell biology Chromatin and nuclear signals Signal transduction Growth factors and hormones Binding protein Cytoskeleton Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 47kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Estrogen Related Receptor alpha: 101-200/423 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.
Subunit:
Binds DNA as a monomer or a homodimer. Interacts (via the AF2 domain) with coactivator PPARGC1A (via the L3 motif); the interaction greatly enhances transriptional activity of genes involved in energy metabolism. Interacts with PIAS4; the interaction enhances sumoylation.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
SWISS:
P11474
Gene ID:
2101
Database links:Entrez Gene: 2101 Human
Entrez Gene: 26379 Mouse
Omim: 601998 Human
SwissProt: P11474 Human
SwissProt: O08580 Mouse
Unigene: 110849 Human
Unigene: 386776 Mouse
Unigene: 130171 Rat
Product Picture Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-ERR-alpha (SL6998R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Sample:
Lovp(Human) Cell Lysate at 30 ug
Primary: Anti- ERR-alpha (SL6998R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ERR-alpha) Polyclonal Antibody, Unconjugated (SL6998R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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