Product Name	Neutrophil Elastase
Chinese Name	中性粒细胞弹性蛋白酶ELANE抗体
Alias	Bone Marrow Serine Protease; ELA 2; ELA2; ELANE; Elastase 2; Elastase-2; Elastase 2 neutrophil; Elastase neutrophil expressed; Elastase-2; ELNE_HUMAN; GE antibody Granulocyte derived elastase; HLE; HNE; Human leukocyte elastase; Leukocyte elastase; Leukocyte Elastase Precursor; Medullasin; NE; Neutrophil elastase; Neutrophill elastase; PMN E; PMN Elastase; Polymorphonuclear elastase; SCN1; Serine protease; SERP1.
literatures	Specific References  (20)     |     SL6982R has been referenced in 20 publications. [IF=20.479] Andrew R. Sas. et al. A new neutrophil subset promotes CNS neuron survival and axon regeneration. Nat Immunol. 2020 Oct;21(12):1496-1505  FC ;  Mouse.   PubMed:33106668 [IF=16.036] Abbas Jarrahi. et al. rhDNase Improves Acute Respiratory Distress Syndrome Via Neutrophil Extracellular Trap Degradation. J THROMB HAEMOST. 2023 May;:  FCM ;  Mouse.   PubMed:37196848 [IF=14.903] Luchen Sun. et al. A novel mesenchymal stem cell-based regimen for acute myeloid leukemia differentiation therapy. ACTA PHARM SIN B. 2023 May;:  WB ;  Human.   PubMed:10.1016/j.apsb.2023.05.007 [IF=13.116] Vaibhav K et al. Neutrophil extracellular traps exacerbate neurological deficits after traumatic brain injury. Sci Adv.2020 May 29;6(22):eaax8847.  FCM ;  Mouse.   PubMed:32523980 [IF=10.245] Qin Tan. et al. Periodontitis pathogen Porphyromonas gingivalis promotes pancreatic tumorigenesis via neutrophil elastase from tumor-associated neutrophils. GUT MICROBES. 2022;14(1):2073785  IF ;  Mouse.   PubMed:35549648 [IF=8.44] Li Xiangdong. et al. Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3. J TRANSL MED. 2023 Dec;21(1):1-19  WB ;  Mouse.   PubMed:36788538 [IF=7.793] Wang H et al. The effect of exposure time and concentration of airborne PM2. 5 on lung injury in mice: A transcriptome analysis. Redox Biol. 2019 Jul 2;26:101264.  IHC-P ;  Mouse.   PubMed:31279222 [IF=7.793] Gao J et al. Metformin protects against PM2. 5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2. Redox Biol. 2019 Oct 19;28:101345.  IHC-P ;  Mouse.   PubMed:31669973 [IF=7.129] Yongcan Wu. et al. Probiotics ameliorates pulmonary inflammation via modulating gut microbiota and rectifying Th17/Treg imbalance in a rat model of PM2.5 induced lung injury. ECOTOX ENVIRON SAFE. 2022 Oct;244:114060  IHC ;  Rat.   PubMed:36115151 [IF=6.02] Wang H et al. AMPKα2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction.Free Radic Biol Med. 2018 Jun;121:202-214.  WB ;  Mouse,Human&Rat.   PubMed:29753072 [IF=5.854] Wang, Xiaobin. et al. Combination regimen of granulocyte colony-stimulating factor and recombinant human thrombopoietin improves the curative effect on elderly patients with leukemia through inducing pyroptosis and ferroptosis of leukemia cells. CANCER GENE THER. 2022 Jun;:1-9  CoIP, WB ;  Human.   PubMed:35768562 [IF=5.23] Arecco, N., et al. "Elastase levels and activity are increased in dystrophic muscle and impair myoblast cell survival, proliferation and differentiation."Scientific Reports 6 (2016): 24708.  IHC-P, WB ;  Mouse.   PubMed:27241590 [IF=4.22] Krishnan, Subramanian, et al. "Serotype O18 avian pathogenic and neonatal meningitis Escherichia coli strains employ similar pathogenic strategies for the onset of meningitis." Virulence 6.8 (2015): 777-786.  IHC-P ;  Mouse.   PubMed:26407066 [IF=4.12] Arecco et al. Elastase levels and activity are increased in dystrophic muscle and impair myoblast cell survival, proliferation and differentiation. (2016) Sci.Rep. 6:24708  IF,WB ;  Mouse.   PubMed:27241590 [IF=3.46] Peng et al. Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease. (2018) J.Biomed.Sci. 25:24  IHC ;  Mouse.   PubMed:29540173 [IF=3.457] Ye, Y. et al. CXCR1/CXCR2 antagonist G31P inhibits nephritis in a mouse model of uric acid nephropathy.(2018) BIOMED PHARMACOTHER  IHC ;  mouse.   PubMed:10.1016/j.biopha.2018.07.077 [IF=2.35] Li, Guofu, et al. "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." International Journal of Molecular Medicine 38.3 (2016): 767-775.  WB ;  Rat.   PubMed:27430552 [IF=2.26] Maekawa, Toshihiro, et al. "Prophylactic Effect of Lactobacillus pentosus strain S-PT84 on Candida Infection and Gastric Inflammation in a Murine Gastrointestinal Candidiasis Model [Errata]." Medical mycology journal 57.4 (2016): E81-E92.  IHC-P ;  Mouse.   PubMed:27904074 [IF=1.704] Störmann P et al. Contributing factors in the development of acute lung injury in a murine double hit model. Eur J Trauma Emerg Surg. 2019 Apr 1.  IHC-P ;  Mouse.   PubMed:30937460 [IF=1.53] Jeffery, Unity, et al. "Dogs Cast NETs too: Canine neutrophil extracellular traps in health and immune-mediated hemolytic anemia." Veterinary Immunology and Immunopathology (2015).  Dog.   PubMed:26574161
Research Area	Cell biology  Signal transduction  Cytoskeleton  Extracellular matrix
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	26kDa
Cellular localization	cytoplasmic Extracellular matrix
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Neutrophil Elastase/ELANE: 101-200/267
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]. Function: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Subunit: Interacts with NOTCH2NL. Tissue Specificity: Bone marrow cells. DISEASE: Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Similarity: Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain. SWISS: P08246 Gene ID: 1991 Database links: Entrez Gene: 1991 Human Entrez Gene: 50701 Mouse Omim: 130130 Human SwissProt: P08246 Human SwissProt: Q3UP87 Mouse Unigene: 99863 Human Unigene: 262194 Mouse
Product Picture	Sample: Spinal cord (Mouse) Lysate at 40 ug Primary: Anti-Neutrophil Elastase (SL6982R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 48 kD Sample: Lane 1: Bone (Mouse) Tissue Lysate at 40 ug Lane 2: Bone (Rat) Tissue Lysate at 40 ug Primary: Anti-Neutrophil Elastase (SL6982R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 40 kD Sample: HL60(Human) Cell Lysate at 30 ug Primary: Anti-Neutrophil Elastase (SL6982R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 41 kD Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Neutrophil Elastase (SL6982R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 31/46 kD Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-Neutrophil Elastase antibody (SL6982R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).

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