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Product Name MIER2 Chinese Name 中胚层诱导早期反应蛋白2抗体 Alias KIAA1193. mesoderm induction early response 1, family member 2; Mesoderm induction early response protein 2; Mi-er2; Mier2; MIER2_HUMAN. Research Area Cell biology transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 60kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen manKLH conjugated synthetic peptide derived from human MIER2: 301-400/545 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail MIER2 is a 545 amino acid protein encoded by a gene that maps to human chromosome 19p13.3. Consisting of around 63 million bases with over 1,400 genes, chromosome 19 makes up over 2% of human genomic DNA. Chromosome 19 includes a diversity of interesting genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin superfamily members including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG family, and Fc?receptors. Key genes for eye color and hair color also map to chromosome 19. Peutz-Jeghers syndrome, spinocerebellar ataxia type 6, the stroke disorder CADASIL, hypercholesterolemia and insulin-dependent diabetes have been linked to chromosome 19. Translocations with chromosome 19 and chromosome 14 can be seen in some lymphoproliferative disorders and typically involve the proto-oncogene BCL3.
Function:
Transcriptional repressor.
Subcellular Location:
Nucleus.
Similarity:
Contains 1 ELM2 domain.
Contains 1 SANT domain.
SWISS:
Q8N344
Gene ID:
54531
Database links:Entrez Gene: 54531 Human
SwissProt: Q8N344 Human
Unigene: 101891 Human
Product Picture Sample:
Ovary (Mouse) Lysate at 40 ug
Primary: Anti-MIER2 (SL6839R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MIER2) Polyclonal Antibody, Unconjugated (SL6839R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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