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Product Name MGEA5 Chinese Name MGEA5抗体 Alias Bifunctional protein NCOAT; Glycoside hydrolase O-GlcNAcase; HAT; HEXC; Hexosaminidase C; Histone acetyltransferase; KIAA0679; MEA5; Meningioma expressed antigen 5; Meningioma-expressed antigen 5; MGEA 5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; NCOAT; Nuclear cytoplasmic O GlcNAcase and acetyltransferase; Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; O-GlcNAcase; OGA; NCOAT_HUMAN. Research Area Tumour Cell biology Signal transduction TumourCell biologyMaker Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 103kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MGEA5: 756-850/916 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008].
Function:
Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3 (By similarity).
Subunit:
Monomer. Binds both acetylated and unacetylated histone H4 tail but acetylation on 'Lys-8' of histone H4 abolishes binding (By similarity).
Subcellular Location:
Isoform 3: Nucleus.
Isoform 1: Cytoplasm.
Tissue Specificity:
Ubiquitous. Shows highest expression in the brain, placenta and pancreas. [PTM] Proteolytically cleaved by caspase-3.
SWISS:
O60502
Gene ID:
10724
Database links:Entrez Gene: 10724 Human
Entrez Gene: 76055 Mouse
Omim: 604039 Human
SwissProt: O60502 Human
SwissProt: Q9EQQ9 Mouse
Unigene: 500842 Human
Unigene: 122725 Mouse
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