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Product Name MAGEA12 Chinese Name 黑色素瘤相关抗原12抗体 Alias Cancer/testis antigen 1.12; Cancer/testis antigen family 1 member 12; CT1.12; MAGAC_HUMAN; MAGE 12 antigen; MAGE-12 antigen; MAGE12; MAGE12 antigen; MAGE12F; MAGE12F antigen; MAGEA12; Melanoma antigen family A 12; Melanoma associated antigen 12; Melanoma-associated antigen 12. Research Area Tumour Cell biology Signal transduction Cell type markers TumourCell biologyMaker Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 35kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MAGEA12: 121-210/314 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Not known, though may play a role in tumor transformation or aspects of tumor progression. Tissue specificity: Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.
Function:
Not known, though may play a role in tumor transformation or aspects of tumor progression.
Tissue Specificity:
Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.
Similarity:
Contains 1 MAGE domain.
SWISS:
P43365
Gene ID:
4111
Database links:Entrez Gene: 4111 Human
Omim: 300177 Human
SwissProt: P43365 Human
Unigene: 169246 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAGEA12) Polyclonal Antibody, Unconjugated (SL6818R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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