Product Name	CHAC1
Chinese Name	阳离子转运调控样蛋白1抗体
Alias	Cation transport regulator like protein 1; Cation transport regulator-like protein 1; ChaC; chac1; CHAC1_HUMAN.
Research Area	Cell biology  Signal transduction  Apoptosis  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	29kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CHAC1: 131-264/264
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Pro-apoptotic component of the unfolded protein response pathway. May mediate the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Function: Pro-apoptotic component of the unfolded protein response pathway. May mediate the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Subcellular Location: Cytoplasm, cytosol. Similarity: elongs to the chaC family. SWISS: Q9BUX1 Gene ID: 79094 Database links: Entrez Gene: 505991 Cow Entrez Gene: 79094 Human Entrez Gene: 69065 Mouse Entrez Gene: 362196 Rat GenBank: NP_077016 Human SwissProt: Q9BUX1 Human SwissProt: Q8R3J5 Mouse SwissProt: B3STU3 Rat Unigene: 155569 Human Unigene: 35083 Mouse Unigene: 23367 Rat
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti-CHAC1 (SL6795R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CHAC1) Polyclonal Antibody, Unconjugated (SL6795R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CHAC1 Polyclonal Antibody, Unconjugated(SL6795R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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