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Product Name KCNK3 Chinese Name 酸敏感钾离子Channel protein3抗体 Alias cTBAK 1; Acid sensitive potassium channel protein TASK 1; Cardiac two pore background K(+) channel; K2p3.1; OAT1; potassium channel, subfamily K, member 3; rTASK; TASK 1; TBAK1; TWIK related acid sensitive K+ channel; Two pore potassium channel KT3.1; KCNK3; TASK; TASK1; PPH4; TASK; TASK-1; TBAK1; KCNK3_HUMAN. Research Area Neurobiology Channel protein The cell membrane受体 Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Chicken, Dog, Cow, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 43kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human KCNK3: 51-150/394 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the superfamily of potassium channel proteins that contain two pore-forming P domains. The encoded protein is an outwardly rectifying channel that is sensitive to changes in extracellular pH and is inhibited by extracellular acidification. Also referred to as an acid-sensitive potassium channel, it is activated by the anesthetics halothane and isoflurane. Although three transcripts are detected in northern blots, there is currently no sequence available to confirm transcript variants for this gene. [provided by RefSeq, Aug 2008].
Function:
pH-dependent, voltage-insensitive, background potassium channel protein. Rectification direction results from potassium ion concentration on either side of the membrane. Acts as an outward rectifier when external potassium concentration is low. When external potassium concentration is high, current is inward.
Subcellular Location:
Membrane; Multi-pass membrane protein (Potential).
Tissue Specificity:
Widespread expression in adult. Strongest expression in pancreas and placenta. Lower expression in brain, lung, prostate, heart, kidney, uterus, small intestine and colon.
Similarity:
Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.
SWISS:
O14649
Gene ID:
3777
Database links:Entrez Gene: 3777 Human
Entrez Gene: 16527 Mouse
Omim: 603220 Human
SwissProt: O14649 Human
SwissProt: Q53SU2 Human
SwissProt: O35111 Mouse
Unigene: 645288 Human
Unigene: 439936 Mouse
Unigene: 15536 Rat
Product Picture Sample: Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-KCNK3 (SL6674R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-KCNK3 (SL6674R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Sample:
Urinary bladder (Mouse) Lysate at 40 ug
Primary: Anti- KCNK3 (SL6674R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 49 kD
Blank control(blue): H9C2 (fixed with 2% paraformaldehyde (10 min)) .
Primary Antibody:Rabbit Anti- KCNK3 antibody(SL6674R), Dilution: 6ug in 100 uL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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