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Product Name HRH2 Chinese Name 组胺受体H2抗体 Alias Gastric receptor 1; Gastric receptor Iv H2R; HGNC:5183; HH2R; Histamine H2 receptor; Histamine receptor H2; HRH2; HRH2_HUMAN. Research Area Cell biology Signal transduction transcriptional regulatory factor Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 40kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human HRH2: 161-259/359 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The HRH2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway.
Function:
The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway (By similarity).
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
P25021
Gene ID:
3274
Database links:Entrez Gene: 3274 Human
Entrez Gene: 15466 Mouse
Omim: 142703 Human
SwissProt: P25021 Human
SwissProt: P97292 Mouse
Unigene: 247885 Human
Unigene: 57243 Mouse
Unigene: 138113 Rat
Product Picture Sample:
Small intestine (Mouse) Lysate at 40 ug
Primary: Anti- HRH2 (SL6664R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HRH2) Polyclonal Antibody, Unconjugated (SL6664R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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