Product Name	HRH1
Chinese Name	组胺受体H1抗体
Alias	Histamine H1 receptor; H1R; HisH1; Histamine receptor H1; Histamine receptor subclass H1; HRH1_HUMAN.
literatures	Specific References  (6)     |     SL6663R has been referenced in 6 publications. [IF=11.685] Kristine Elisabeth Eberhard. et al. Neurotransmitter and Neurotransmitter Receptor Expression in the Saccule of the Human Vestibular System. Prog Neurobiol. 2022 Jan;:102238  IHC ;  Human.   PubMed:35104536 [IF=6.038] Liao, Xiaodan. et al. Fullerene nanoparticles for the treatment of ulcerative colitis. 2021 Nov 02  WB ;  Rat.   PubMed:34735681 [IF=3.263] Aya Sato. et al. Histamine depolarizes rat intracardiac ganglion neurons through the activation of TRPC non-selective cation channels. Eur J Pharmacol. 2020 Nov;886:173536  WB ;  Rat.   PubMed:32896550 [IF=2.629] Yu Wen-Yan. et al. Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation. Evid-Based Compl Alt. 2022;2022:4571981  WB ;  Rat.   PubMed:35069759 [IF=1.54] Møller, M. Nue, et al. "Expression of histamine receptors in the human endolymphatic sac: the molecular rationale for betahistine use in Menieres disease." European Archives of Oto-Rhino-Laryngology: 1-6.  IHC-P ;  Human.   PubMed:26208913 [IF=1.504] Maheswari DU et al. Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo. South African Journal of Botany, 2020, 130, 130–140.  WB ;  Mouse.   PubMed:doi:10.1016/j.sajb.2019.12.012
Research Area	Cell biology  immunology  Signal transduction  Growth factors and hormones  G protein-coupled receptor  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, Pig, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	54kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human HRH1: 141-240/487 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]. Function: In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. Subcellular Location: Cell membrane; Multi-pass membrane protein. Post-translational modifications: Potential sites of phosphorylation in the third cytoplasmic loop may play an important role in regulating signal transduction through the receptor molecule. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P35367 Gene ID: 3269 Database links: Entrez Gene: 3269 Human Entrez Gene: 15465 Mouse Entrez Gene: 24448 Rat Omim: 600167 Human SwissProt: P35367 Human SwissProt: P70174 Mouse SwissProt: P31390 Rat Unigene: 1570 Human Unigene: 333327 Mouse Unigene: 177519 Rat Unigene: 81032 Rat
Product Picture	Sample: LOVO(Human) Cell Lysate at 30 ug Primary: Anti-HRH1 (SL6663R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD Sample: Lane 1: U87MG (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Lane 3: Huvec (Human) Cell Lysate at 30 ug Lane 4: Hela (Human) Cell Lysate at 30 ug Primary: Anti-HRH1 (SL6663R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HRH1) Polyclonal Antibody, Unconjugated (SL6663R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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