Product Name	GATA2
Chinese Name	GATABinding protein2抗体
Alias	GATA-2; GATA 2; GATA Binding Protein 2; GATA-binding protein 2; Gata2; GATA2_HUMAN; MGC2306; NFE 1B; NFE1B.
literatures	Specific References  (2)     |     SL6616R has been referenced in 2 publications. [IF=3.87] Ikari, Akira, et al. "Hyperosmolarity‐Induced Down‐Regulation of Claudin‐2 Mediated by Decrease in PKCβ‐Dependent GATA‐2 in MDCK Cells." Journal of Cellular Physiology (2015).  WB ;  Dog.   PubMed:25825272 [IF=3.15] Yang Zhai. et al. Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma. Bmc Cancer. 2021 Dec;21(1):1-13  IHC ;  Human.   PubMed:33648465
Research Area	Tumour  Cardiovascular  Signal transduction  Stem cells  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	(predicted: Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	53kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GATA-2: 351-450/480
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites. Function: Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. Subcellular Location: Nucleus. Tissue Specificity: Endothelial cells. DISEASE: Defects in GATA2 are the cause of dendritic cell monocyte lymphocyte B and natural killer lymphocyte deficiency (DCML) [MIM:614172]. DCML is an immunodeficiency disease characterized by profoundly decreased or absent monocytes, B-lymphocytes, natural killer lymphocytes, and circulating and tissue dendritic cells, with little or no effect on T-cell numbers. Clinical features of DCML include susceptibility to disseminated non-tuberculous mycobacterial infections, papillomavirus infections, opportunistic fungal infections, and pulmonary alveolar proteinosis. Bone marrow hypocellularity and dysplasia of myeloid, erythroid, and megakaryocytic lineages are present in most patients, as are karyotypic abnormalities, including monosomy 7 and trisomy 8. Similarity: Contains 2 GATA-type zinc fingers. SWISS: P23769 Gene ID: 2624 Database links: Entrez Gene: 416018 Chicken Entrez Gene: 2624 Human Entrez Gene: 14461 Mouse Entrez Gene: 25159 Rat Omim: 137295 Human SwissProt: P23824 Chicken SwissProt: P23769 Human SwissProt: O09100 Mouse SwissProt: Q924Y4 Rat Unigene: 367725 Human Unigene: 34322 Rat
Product Picture	Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Mouse Liver tissue lysates Primary: Anti-GATA2 (SL6616R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kDa Observed band size: 55 kDa Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GATA2) Polyclonal Antibody, Unconjugated (SL6616R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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