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Product Name Galectin 1 Chinese Name 半乳糖凝集素1抗体 Alias 14 kDa laminin-binding protein; 14 kDa lectin; Beta galactoside binding lectin; Beta galactoside binding lectin L 14 I; beta galactoside binding protein; Beta-galactoside-binding lectin L-14-I; Gal 1; Gal-1; Galaptin; Galbp; Galectin; Galectin-1;Galectin1; GBP; HBL; HLBP14; HPL; L 14.5; L-14.5; L14; Lactose binding lectin 1; Lactose-binding lectin 1; Lect14; Lectin galactoside binding soluble 1;Lectin galactoside-binding soluble 1; LEG1_HUMAN; LGALS 1; LGALS1; Lgals1 lectin galactose binding soluble 1; MAPK activating protein MP12; Putative MAPK activating protein MP12; Putative MAPK-activating protein PM12; S Lac lectin 1; S-Lac lectin 1. literatures Specific References (1) | SL6594R has been referenced in 1 publications.[IF=3.345] Xu et al. Morphological and proteomic analyses reveal that unsaturated guluronate oligosaccharide modulates multiple functional pathways in murine macrophage RAW264.7 cells. (2015) Mar.Drug. 13:1798-818 WB ; Mouse.Research Area Cell biology Signal transduction Apoptosis Cyclin Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 15kDa Cellular localization Extracellular matrix Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Galectin 1: 65-135/135 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008].
Function:
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
Subunit:
Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N-acetyllactosamine).
Subcellular Location:
Secreted, extracellular space, extracellular matrix.
Tissue Specificity:
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
Similarity:
Contains 1 galectin domain.
SWISS:
P09382
Gene ID:
3956
Database links:Entrez Gene: 3956 Human
Entrez Gene: 16852 Mouse
Omim: 150570 Human
SwissProt: P09382 Human
SwissProt: P16045 Mouse
Unigene: 445351 Human
Unigene: 43831 Mouse
Unigene: 57 Rat
Product Picture Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-Galectin 1 (SL6594R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 14 kD
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Galectin-1 Polyclonal Antibody, Unconjugated(SL6594R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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