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Product Name Testin Chinese Name Tumour抑制基因Testin抗体 Alias Testin; TES; TESS 2; TESS; TESS2; TESS-2; Testis derived transcript (3 LIM domains); Testis derived transcript; TES_HUMAN. Research Area Tumour Signal transduction transcriptional regulatory factor Zinc finger protein Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 48kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Testin: 161-210/421 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Cancer-associated chromosomal changes often involveregions containing fragile sites. This gene maps to a commomfragile site on chromosome 7q31.2 designated FRA7G. This gene issimilar to mouse Testin, a testosterone-responsive gene encoding aSertoli cell secretory protein containing three LIM domains. LIMdomains are double zinc-finger motifs that mediate protein-proteininteractions between transcription factors, cytoskeletal proteinsand signaling proteins. This protein is a negative regulator ofcell growth and may act as a tumor suppressor. This scaffoldprotein may also play a role in cell adhesion, cell spreading andin the reorganization of the actin cytoskeleton. Multiple proteinisoforms are encoded by transcript variants of this gene.
Function:
Scaffold protein that may play a role in cell adhesion,cell spreading and in the reorganization of the actin cytoskeleton.Plays a role in the regulation of cell proliferation. May act as atumor suppressor. Inhibits tumor cell growth.
Subunit:
Interacts via LIM domain 1 with ZYX. Interacts (via LIMdomain 3) with ENAH and VASP. Interacts with talin, actin,alpha-actinin, GRIP1 and PXN. Interacts (via LIM domain 2) withACTL7A (via N-terminus). Heterodimer with ACTL7A; the heterodimerinteracts with ENAH to form a heterotrimer.
Subcellular Location:
Cytoplasm. Cell junction, focal adhesion.Note=Detected along actin stress fibers.
Tissue Specificity:
Ubiquitous.
Similarity:
Belongs to the prickle / espinas / testin family.
Contains 3 LIM zinc-binding domains.
Contains 1 PET domain.
SWISS:
Q9UGI8
Gene ID:
26136
Database links:
Entrez Gene: 26136 Human
Entrez Gene: 21753 Mouse
Omim: 606085 Human
SwissProt: Q2QLB2 Horse
SwissProt: Q9UGI8 Human
SwissProt: P47226 Mouse
Unigene: 592286 Human
Unigene: 664957 Human
Unigene: 436548 Mouse
Unigene: 44847 Rat
Product Picture Sample: Lung (Mouse) Lysate at 40 ug
Primary: Anti-Testin (SL6502R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Testin Polyclonal Antibody, Unconjugated(SL6502R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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