Product Name	caspase-8 subunit p18
Chinese Name	半胱氨酸蛋白酶8抗体
Alias	Caspase-8 subunit p18; ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch 5; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP 4; CAP4; CASP 8; CASP-8; CASP8; CASP8_HUMAN; Caspase 8; Caspase 8 apoptosis related cysteine peptidase; Caspase-8 subunit p18; Caspase8; CED 3; FADD homologous ICE/CED 3 like protease; FADD Homologous ICE/CED3 Like Protease; FADD Like ICE; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE like apoptotic protease 5; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH; MACH beta 1/2/3/4 protein; MCH 5; MCH5; MGC78473; MORT1 associated CED 3 homolog; MORT1 associated CED3 homolog; MORT1-associated CED-3 homolog; OTTHUMP00000163717; OTTHUMP00000163720; OTTHUMP00000163724; OTTHUMP00000163725; OTTHUMP00000165062; OTTHUMP00000165063; OTTHUMP00000165064; OTTHUMP00000206552; OTTHUMP00000206582
literatures	Specific References  (7)     |     SL6463R has been referenced in 7 publications. [IF=5.572] Jiayuan Luo. et al. Effects of saponins isolated from Polygonatum sibiricum on H2O2-induced oxidative damage in RIN-m5F cells and its protective effect on pancreas. FOOD CHEM TOXICOL. 2023 May;175:113724  WB,FCM ;  Rat.   PubMed:36935075 [IF=3.547] You XG et al. Phenylephrine Induces Necroptosis and Apoptosis in Corneal Epithelial Cells Dose-and Time-Dependently. Toxicology. 2019 Oct 9;428:152305.  ELISA ;  Human.   PubMed:31605733 [IF=3.07] Meneses, Carla, et al. "The angiotensin-(1–7)/Mas axis reduces myonuclear apoptosis during recovery from angiotensin II-induced skeletal muscle atrophy in mice." Pflügers Archiv-European Journal of Physiology (2014): 1-10.  WB ;  Mouse.   PubMed:25292283 [IF=2.9] Shan, Ming, and Ting-Jun Fan. "Cytotoxicity of carteolol to human corneal epithelial cells by inducing apoptosis via triggering the Bcl-2 family protein-mediated mitochondrial pro-apoptotic pathway." Toxicology in Vitro (2016).  ELISA ;  Human.   PubMed:27216471 [IF=2.72] Sudeshna Nandi. et al. Anti-cancer effect of astrakurkurol from a folklore tribal mushroom on human hepatocellular carcinoma cells via mediating cell cycle inhibition, apoptosis, and migration. 2021 Nov 22  WB ;  Human.   PubMed:34811765 [IF=2.133] Cetintas, Vildan Bozok, et al. "Effects of flavopiridol on critical regulation pathways of CD133high/CD44high lung cancer stem cells." Medicine 95.43 (2016): e5150.  IF(ICC) ;  Mouse.   PubMed:27787370 [IF=1.72] Zhao, Jun, et al. "The cytotoxic and pro-apoptotic effects of phenylephrine on corneal stromal cells via a mitochondrion-dependent pathway both in vitro and in vivo." Experimental and Toxicologic Pathology (2016).  ELISA ;  Human.   PubMed:27344612
Research Area	Cell biology  immunology  Neurobiology  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /Test IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	18/55kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human caspase-8 subunit p18: 188-280/479
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Initiator caspases, which include caspase-8, activate effector caspases by cleaving inactive forms of effector caspases. In the activation cascade responsible for apoptosis induced by TNFRSF1A and mediated by TNFRSF6/FAS, caspase-8 is the most upstream protease. Caspase-8 binds to adaptor molecule FADD, forming an aggregate referred to as death-inducing signaling complex (DISC), which activates caspase-8. The actived protein is released from the complex and further activates downstream apoptotic proteases. Caspase-8, which is a heterodimer consisting of two subunits (p18 and p10), is widely expressed, but is detected at highest levels in peripheral blood leukocytes (PBLs), thymus, liver and spleen. Defects in CASP8, the gene encoding for caspase-8, may cause CASP8D (caspase-8 deficiency disorder), which is characterized by splenomegaly and CD95-induced apoptosis of PBLs, and may lead to immunodeficiency due to defects in T lymphocyte, NK cell and B lymphocyte activation. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2. Subcellular Location: Cytoplasm. Tissue Specificity: Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. Similarity: Belongs to the peptidase C14A family. Contains 2 DED (death effector) domains. SWISS: Q14790 Gene ID: 841 Database links: Entrez Gene: 841 Human Entrez Gene: 12370 Mouse Entrez Gene: 54474 Rat Entrez Gene: 64044 Rat Omim: 601763 Human SwissProt: Q14790 Human SwissProt: O89110 Mouse SwissProt: Q9JHX4 Rat Unigene: 599762 Human Unigene: 655983 Human Unigene: 336851 Mouse
Product Picture	Sample: Raji Cell (Human) Lysate at 40 ug Hela Cell (Human) Lysate at 40 ug Primary: Anti-caspase-8 subunit p18 (SL6463R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18/55 kD Observed band size: 55 kD Sample: Hela-UV(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Jurkat-UV(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug A431-UV(Human) Cell Lysate at 30 ug Molt-4(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug 293T-UV(Human) Cell Lysate at 30 ug U87MG(Human) Cell Lysate at 30 ug U251-UV(Human) Cell Lysate at 30 ug Primary: Anti-caspase-8 subunit p18 (SL6463R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57/55/43/41/18 kD Observed band size: 57 kD Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (caspase-8 subunit p18) Polyclonal Antibody, Unconjugated (SL6463R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control (blue line): U251 (fixed with 70% ethanol overnight at 4℃ and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-caspase-8 subunit p18 antibody (SL6463R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

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