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Product Name caspase-8 subunit p18 Chinese Name 半胱氨酸蛋白酶8抗体 Alias Caspase-8 subunit p18; ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch 5; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP 4; CAP4; CASP 8; CASP-8; CASP8; CASP8_HUMAN; Caspase 8; Caspase 8 apoptosis related cysteine peptidase; Caspase-8 subunit p18; Caspase8; CED 3; FADD homologous ICE/CED 3 like protease; FADD Homologous ICE/CED3 Like Protease; FADD Like ICE; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE like apoptotic protease 5; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH; MACH beta 1/2/3/4 protein; MCH 5; MCH5; MGC78473; MORT1 associated CED 3 homolog; MORT1 associated CED3 homolog; MORT1-associated CED-3 homolog; OTTHUMP00000163717; OTTHUMP00000163720; OTTHUMP00000163724; OTTHUMP00000163725; OTTHUMP00000165062; OTTHUMP00000165063; OTTHUMP00000165064; OTTHUMP00000206552; OTTHUMP00000206582 literatures Specific References (7) | SL6463R has been referenced in 7 publications.Research Area Cell biology immunology Neurobiology Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /Test IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 18/55kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human caspase-8 subunit p18: 188-280/479 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Initiator caspases, which include caspase-8, activate effector caspases by cleaving inactive forms of effector caspases. In the activation cascade responsible for apoptosis induced by TNFRSF1A and mediated by TNFRSF6/FAS, caspase-8 is the most upstream protease. Caspase-8 binds to adaptor molecule FADD, forming an aggregate referred to as death-inducing signaling complex (DISC), which activates caspase-8. The actived protein is released from the complex and further activates downstream apoptotic proteases. Caspase-8, which is a heterodimer consisting of two subunits (p18 and p10), is widely expressed, but is detected at highest levels in peripheral blood leukocytes (PBLs), thymus, liver and spleen. Defects in CASP8, the gene encoding for caspase-8, may cause CASP8D (caspase-8 deficiency disorder), which is characterized by splenomegaly and CD95-induced apoptosis of PBLs, and may lead to immunodeficiency due to defects in T lymphocyte, NK cell and B lymphocyte activation.
Subunit:
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
Similarity:
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
SWISS:
Q14790
Gene ID:
841
Database links:Entrez Gene: 841 Human
Entrez Gene: 12370 Mouse
Omim: 601763 Human
SwissProt: Q14790 Human
SwissProt: O89110 Mouse
Unigene: 599762 Human
Unigene: 655983 Human
Unigene: 336851 Mouse
Product Picture Sample:
Raji Cell (Human) Lysate at 40 ug
Hela Cell (Human) Lysate at 40 ug
Primary: Anti-caspase-8 subunit p18 (SL6463R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18/55 kD
Observed band size: 55 kD
Sample:
Hela-UV(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Jurkat-UV(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
A431-UV(Human) Cell Lysate at 30 ug
Molt-4(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
293T-UV(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
U251-UV(Human) Cell Lysate at 30 ug
Primary: Anti-caspase-8 subunit p18 (SL6463R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57/55/43/41/18 kD
Observed band size: 57 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (caspase-8 subunit p18) Polyclonal Antibody, Unconjugated (SL6463R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Blank control (blue line): U251 (fixed with 70% ethanol overnight at 4℃ and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-caspase-8 subunit p18 antibody (SL6463R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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