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Product Name PCNP Chinese Name PEST含核蛋白抗体 Alias PCNP; PCNP_HUMAN; PEST containing nuclear protein; PEST proteolytic signal containing nuclear protein; PEST proteolytic signal-containing nuclear protein; PEST-containing nuclear protein. Research Area Cell biology Chromatin and nuclear signals Cyclin Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 19kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PCNP: 21-120/178 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail PCNP is a novel 178 amino acid nuclear protein implied to play a role in cell cycle regulation and tumorigenesis. PCNP is ubiquitinated post-translationally by NIRF (Np95/ICBP90-like RING finger protein), a ubiquitin ligase. Existing as three isoforms produced by alternative splicing events, PCNP is encoded by a gene mapping to human chromosome 3q12.3. Chromosome 3 houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3
Function:
May be involved in cell cycle regulation.
Subunit:
Interacts with UHRF2/NIRF.
Post-translational modifications:
Ubiquitinated; mediated by UHRF2 and leading to its subsequent proteasomal degradation.
SWISS:
Q8WW12
Gene ID:
57092
Database links:Entrez Gene: 57092 Human
Entrez Gene: 76302 Mouse
SwissProt: Q8WW12 Human
SwissProt: Q6P8I4 Mouse
Unigene: 275865 Human
Unigene: 202642 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNP) Polyclonal Antibody, Unconjugated (SL6392R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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