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Product Name BRF1 Chinese Name RNA聚合酶III抗体 Alias B related factor 1; B-related factor 1; BRF; BRF-1; brf1; brfu; General transcription factor IIIB, 90kD subunit; GTF3B; hBRF; hTFIIIB90; RNA polymerase III; subunit 2; TAF3B2; TAF3C; TATA box-binding protein-associated factor; TF3B_HUMAN; TFIIIB90; TFIIIB90-1; TFIIIB90 1; Transcription factor IIIB 90 kDa subunit; Transcription initiation factor IIIB. Research Area Cell biology Chromatin and nuclear signals Signal transduction Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 74kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from Human BRF1/RNA polymerase III: 501-600/677 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail responsible for the synthesis of a different class of RNA. RNA polymerase I (Pol I) transcribes the rRNA (ribosomal RNA) genes for the precursor of the 28S, 18S, and 5.8S molecules of the ribosome. RNA polymerase II transcribes protein-encoding genes into mRNA (messenger RNA) and snRNA (small nuclear RNA) genes into snRNAs that influence the processing of other classes of RNA. RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes. Each class of RNA polymerase is assembled from 9 to 15 different polypeptides. The RPB6 and RPB8 subunits are shared by all 3 RNA polymerases.
Function:
General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
Subcellular Location:
Nucleus.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.
SWISS:
Q92994
Gene ID:
2972
Database links:Entrez Gene: 2972 Human
Omim: 604902 Human
SwissProt: Q92994 Human
Unigene: 424484 Human
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