Product Name	IL-1 Beta
Chinese Name	白介素1β抗体
Alias	IL1B_HUMAN; Interleukin-1 beta; interleukin 1 beta; IL1F2; IL-1 beta; Catabolin; IL-1; IL1F2; IL1beta; IL1-BETA; IL-1beta; IL-1 Beta; IL-1β; IL1β; IL-1B; IL1B;
literatures	Specific References  (30)     |     SL6319R has been referenced in 30 publications. [IF=13.116] Zhang X et al. Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer’s diseaseSci Adv.2020 Dec 4;6(49):eabb8680.  IF ;  Mouse.   PubMed:33277246 [IF=11.799] Chenyu Zhang. et al. The Nrf2-NLRP3-caspase-1 axis mediates the neuroprotective effects of Celastrol in Parkinson's disease. Redox Biol. 2021 Nov;47:102134  WB ;  mosue.   PubMed:34600334 [IF=7.238] Chinthalapally V. Rao. et al. GSK3‐ARC/Arg3.1 and GSK3‐Wnt signaling axes trigger amyloid‐β accumulation and neuroinflammation in middle‐aged Shugoshin 1 mice. Aging Cell. 2020 Oct;19(10):e13221  IHC ;  Mouse.   PubMed:32857910 [IF=6.656] Vittoria Borgonetti. et al. Zingiberene, a non-zinc-binding class I HDAC inhibitor: A novel strategy for the management of neuropathic pain. PHYTOMEDICINE. 2023 Mar;111:154670  WB ;  Mouse.   PubMed:36681053 [IF=6.306] Zhou P et al. Histamine-4 receptor antagonist JNJ7777120 inhibits pro-inflammatory microglia and prevents the progression of Parkinson-like pathology and behaviour in a rat model.(2018)Brain Behav. Immun. Nov 05.  WB ;  Rat.   PubMed:30408497 [IF=6.208] Chao Huang. et al. Loss of TRIM67 Attenuates the Progress of Obesity-Induced Non-Alcoholic Fatty Liver Disease. INT J MOL SCI. 2022 Jan;23(13):7475  WB ;  Mouse.   PubMed:35806477 [IF=5.714] Yue Xiong. et al. Thymol improves autism-like behaviour in VPA-induced ASD rats through the Pin1/p38 MAPK pathway. INT IMMUNOPHARMACOL. 2023 Apr;117:109885  WB ;  Rat.   PubMed:36842231 [IF=5.682] Wu, Mengyu. et al. PTP1B Inhibitor Claramine Rescues Diabetes-Induced Spatial Learning and Memory Impairment in Mice. MOL NEUROBIOL. 2022 Nov;:1-21  WB ;  Mouse.   PubMed:36319905 [IF=5.194] Yu-Shen Luo. et al. Acrylonitrile exposure triggers ovarian inflammation and decreases oocyte quality probably via mitochondrial dysfunction induced apoptosis in mice. CHEM-BIOL INTERACT. 2022 Jun;360:109934  WB ;  Mouse.   PubMed:35429547 [IF=4.966] Wei‐Cheng Chen. et al. Resistin enhances IL‐1β and TNF‐α expression in human osteoarthritis synovial fibroblasts by inhibiting miR‐149 expression via the MEK and ERK pathways. Faseb J. 2020 Oct;34(10):13671-13684  WB,IHC ;  Human.   PubMed:32790946 [IF=4.858] Kun Hao. et al. Targeting BRD4 prevents acute gouty arthritis by regulating pyroptosis. Int J Biol Sci. 2020; 16(16): 3163–3173  WB ;  Rat, Human.   PubMed:33162822 [IF=4.691] Dai-Chao Maet al. Kv1.3 channel blockade alleviates cerebral ischemia/reperfusion injury by reshaping M1/M2 phenotypes and compromising the activation of NLRP3 inflammasome in microglia. Exp Neurol . 2020 Oct;332:113399.  WB ;  rat.   PubMed:32652099 [IF=4.13] Fukuda M et al. Disruption of P2X4 purinoceptor and suppression of the inflammation associated with cerebral aneurysm formation. J Neurosurg. 2019 Dec 20:1-13.  WB ;  Mouse.   PubMed:31860812 [IF=3.921] Meiyun Cai. et al. Kaemperfol alleviates pyroptosis and microglia-mediated neuroinflammation in Parkinson's disease via inhibiting p38MAPK/NF-κB signaling pathway. Neurochem Int. 2021 Nov;:105221  WB ;  Rat.   PubMed:34780806 [IF=3.895] Yang W et al. The Effect of Low and High Dose Deoxynivalenol on Intestinal Morphology, Distribution, and Expression of Inflammatory Cytokines of Weaning Rabbits. Toxins (Basel). 2019 Aug 13;11(8). pii: E473.  WB&IHC-P ;  Rabbit.   PubMed:31412640 [IF=3.895] Sun D et al. Canonical Transient Receptor Potential Channel 3 Contributes to Febrile Seizure Inducing Neuronal Cell Death and Neuroinflammation.Cell. Mol. Neurobiol. 2018 Aug;38(6), 1215–1226.  IF ;  Rat.   PubMed:29748835 [IF=3.69] Dai-Chao Ma. et al. Salvianolic Acids for Injection alleviates cerebral ischemia/reperfusion injury by switching M1/M2 phenotypes and inhibiting NLRP3 inflammasome/pyroptosis axis in microglia in vivo and in vitro. J Ethnopharmacol. 2021 Apr;270:113776  WB ;  Rat.   PubMed:33421597 [IF=3.412] Abdelaziz Adam Idriss Arbab. et al. Metformin Inhibits Lipoteichoic Acid–Induced Oxidative Stress and Inflammation Through AMPK/NRF2/NF-κB Signaling Pathway in Bovine Mammary Epithelial Cells. Front Vet Sci. 2021; 8: 661380  WB ;  Bovine.   PubMed:34262962 [IF=3.17] Yu H et al. Echinocystic acid, a natural plant extract, alleviates cerebral ischemia/reperfusion injury via inhibiting the JNK signaling pathway. Eur J Pharmacol. 2019 Oct 15;861:172610.  WB ;  Mouse.   PubMed:31425684 [IF=3.099] Ayano Nakai. et al. Development of a method to identify persistent and blanchable redness by skin blotting in mice. INT WOUND J. 2022 Nov;:  IHC ;  Mouse.   PubMed:36367160 [IF=2.985] Mengmeng Yang. et al. Mesenchymal stem cell-conditioned medium improved mitochondrial function and alleviated inflammation and apoptosis in non-alcoholic fatty liver disease by regulating SIRT1. Biochem Bioph Res Co. 2021 Mar;546:74  WB ;  Mouse, Human.   PubMed:33578292 [IF=2.882] Gul Fatma Yarim. et al. Nobiletin attenuates inflammation via modulating proinflammatory and antiinflammatory cytokine expressions in an autoimmune encephalomyelitis mouse model. Fitoterapia. 2022 Jan;156:105099  IHC ;  Rat.   PubMed:34896483 [IF=2.695] Jaclyn Iannucci. et al. Isoform-Specific Effects of Apolipoprotein E on Markers of Inflammation and Toxicity in Brain Glia and Neuronal Cells In Vitro. Curr Issues Mol Biol. 2021 Jun;43(1):215-225  WB ;  Human.   PubMed:34071762 [IF=2.55] Dinesh, Palani, and MahaboobKhan Rasool. "Berberine, an isoquinoline alkaloid suppresses TXNIP mediated NLRP3 inflammasome activation in MSU crystal stimulated RAW 264.7 macrophages through the upregulation of Nrf2 transcription factor and alleviates MSU crystal induced inflammation in rats." International Immunopharmacology 44 (2017): 26-37.  WB ;  Mouse.   PubMed:28068647 [IF=2.376] Zheng Xue. et al. Gastrodin improves neuroinflammation-induced cognitive dysfunction in rats by regulating NLRP3 inflammasome. BMC ANESTHESIOL. 2022 Dec;22(1):1-13  WB ;  Rat.   PubMed:36456961 [IF=2.33] Li, Kang, et al. "CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts." Molecular Medicine Reports.  WB ;  Human.   PubMed:23338226 [IF=1.41] Han JK et al. Anti-inflammatory effect of polydeoxyribonucleotide on zoledronic acid-pretreated and lipopolysaccharide-stimulated RAW 264.7 cells. (2018) Exp.Ther.Med. 16:400-405  WB ;  human.   PubMed:29896266 [IF=1.25] Mehmet Balbaba. et al. Anti-inflammatory effect of cortistatin in rat endotoxin-induced uveitis model. Indian J Ophthalmol. 2020 Sep; 68(9): 1920–1924  IHC ;  Rat.   PubMed:32823415 [IF=0] Yamada H et al. LATE-ONSET ALZHEIMER'S DISEASE ANIMAL MODEL AND USES THEREOF. US Patent20190387723  WB, IHC-P&IHF ;  Mouse.   PubMed:US Patent20190387723 [IF=0] Evashwick-Rogler et al. Inhibiting tumor necrosis factor-alpha at time of induced intervertebral disc injury limits long-term pain and degeneration in a rat model. (2018) JOR.Spine. 1  IHC ;  Rat.   PubMed:29963655
Research Area	Cell biology  immunology  Growth factors and hormones  glycoprotein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	17/32kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human IL-1 Beta: 161-269/269
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]. Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Subunit: Monomer. Subcellular Location: Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Similarity: Belongs to the IL-1 family. SWISS: P01584 Gene ID: 3553 Database links: Entrez Gene: 3553 Human Entrez Gene: 16176 Mouse Entrez Gene: 24494 Rat Omim: 147720 Human SwissProt: P01584 Human SwissProt: P10749 Mouse SwissProt: Q63264 Rat Unigene: 126256 Human Unigene: 222830 Mouse Unigene: 9869 Rat
Product Picture	Sample: Brain (Mouse) Lysate at 40 ug Intestine (Mouse) Lysate at 40 ug Primary: Anti-IL-1 Beta (SL6319R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 17/30 kD Observed band size: 30 kD Sample: Raw264.7(Mouse) Cell Lysate at 30 ug Liver (Mouse) Lysate at 40 ug Primary: Anti-IL-1 Beta (SL6319R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/30 kD Observed band size: 30 kD Sample: Lane 1: Recombinant mouse IL-1b protein, C-His Primary: Anti-IL-1 Beta (SL6319R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/32 kDa Observed band size: 25 kDa Sample: Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti- IL-1 Beta (SL6319R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/30 kD Observed band size: 30 kD Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1 Beta) Polyclonal Antibody, Unconjugated (SL6319R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-IL-1 Beta antibody (SL6319R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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