Product Name	Prostaglandin E Receptor EP1
Chinese Name	前列腺素EP1受体抗体
Alias	Prostaglandin E Receptor EP1; EP1; PGE receptor EP1 subtype; PGE2 receptor EP1 subtype; Prostaglandin E receptor 1 subtype EP1 42kDa; Prostaglandin E receptor 1 subtype EP1; Prostaglandin E2 receptor EP1 subtype; Prostanoid EP1 receptor; PE2R1_HUMAN.
literatures	Specific References  (3)     |     SL6316R has been referenced in 3 publications. [IF=4.872] Wu F et al. Manganese exposure caused reproductive toxicity of male mice involving activation of GnRH secretion in the hypothalamus by prostaglandin E2 receptors EP1 and EP2. Ecotoxicol Environ Saf . 2020 Sep 15;201:110712.  WB ;  Mouse.   PubMed:32502905 [IF=3.23] Takemiya et al. Microsomal Prostaglandin E Synthase-1 Facilitates an Intercellular Interaction between CD4⁺ T Cells through IL-1β Autocrine Function in Experimental Autoimmune Encephalomyelitis. (2017) Int.J.Mol.Sci. 18  IHC-F ;  Mouse.   PubMed:29257087 [IF=2.253] Chen Z et al. Seasonal expressions of prostaglandin E synthases and receptors in the prostate of the wild ground squirrel (Spermophilus dauricus). Prostaglandins and Other Lipid Mediators 148 (2020) 106412.  IHC-P ;  squirrel.   PubMed:doi:10.1016/j.prostaglandins.2020.106412
Research Area	Cell biology  Signal transduction  transcriptional regulatory factor  G protein-coupled receptor  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	42kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Prostaglandin E Receptor EP1: 61-160/402 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	PTGER1 is a subtype 1 receptor for prostaglandin E2 (PGE2). This receptor is coupled to the phosphatidylinositol-calcium second messenger system by G(q) proteins. PTGER1 may be an important modulator of renal function and is implicated in the smooth muscle contractile response to PGE2 in various tissues. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Abundant in kidney. Lower level expression in lung, skeletal muscle and spleen, lowest expression in testis and not detected in liver brain and heart. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P34995 Gene ID: 5731 Database links: Entrez Gene: 5731 Human Entrez Gene: 19216 Mouse Entrez Gene: 25637 Rat Omim: 176802 Human SwissProt: P34995 Human SwissProt: P35375 Mouse SwissProt: P70597 Rat Unigene: 159360 Human Unigene: 474430 Mouse Unigene: 11423 Rat
Product Picture	Sample: Brain (Rat) Lysate at 40 ug Primary: Anti-Prostaglandin E Receptor EP1 (SL6316R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Prostaglandin E Receptor EP1 (SL6316R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 45 kD Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PTGER1 Polyclonal Antibody, Unconjugated(SL6316R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Blank control (Black line): Mouse blood (Black). Primary Antibody (green line): Rabbit Anti-Prostaglandin E Receptor EP1 antibody (SL6316R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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