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Product Name DUSP3 Chinese Name 丝氨酸/苏氨酸特定蛋白磷酸酶抗体 Alias Dual specificity phosphatase 3; Dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; DUS3_HUMAN; DUSP3; Serine/threonine specific protein phosphatase; Vaccinia H1-related phosphatase; Vaccinia virus phosphatase VH1 related; VHR. Research Area Tumour Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Pig, Cow, Horse, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 20kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human VHR/DUSP3: 41-140/185 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1.
Function:
Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.
Subunit:
Interacts with VRK3, which seems to activate it's phosphatase activity
Subcellular Location:
Nucleus.
Similarity:
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 tyrosine-protein phosphatase domain.
SWISS:
P51452
Gene ID:
1845
Database links:Entrez Gene: 1845 Human
Entrez Gene: 72349 Mouse
GenBank: NP_004081.1 Human
Omim: 600183 Human
SwissProt: P51452 Human
SwissProt: Q9D7X3 Mouse
Unigene: 181046 Human
Unigene: 196295 Mouse
Unigene: 8286 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DUSP3) Polyclonal Antibody, Unconjugated (SL6246R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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