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Product Name TM4SF3 Chinese Name Transmembrane protein4超家族成员3抗体 Alias TSPAN8; CO029; Tetraspanin 8; Tetraspanin-8; TM4SF3; Transmembrane 4 superfamily member 3; Tspan 8; TSPAN8; Tumor associated antigen CO 029; TSN8_HUMAN. literatures Specific References (1) | SL6241R has been referenced in 1 publications.[IF=4.02] Bhansali, Meenakshi, Jun Zhou, and Lirim Shemshedini. "TM4SF3 and AR: A Nuclear Complex that Stabilizes Both Proteins." Molecular Endocrinology(2016): me-2015. WB ; Human.Research Area Tumour Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 26kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TM4SF3: 101-200/237 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail TM4SF3 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TM4SF3 is known to complex with integrins. Its gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for TM4SF3 gene.
Subcellular Location:
Membrane; Multi-pass membrane protein.
Tissue Specificity:
Gastric, colon, rectal, and pancreatic carcinomas.
Similarity:
Belongs to the tetraspanin (TM4SF) family.
SWISS:
P19075
Gene ID:
7103
Database links:Entrez Gene: 7103 Human
Omim: 600769 Human
SwissProt: P19075 Human
Unigene: 170563 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TM4SF3) Polyclonal Antibody, Unconjugated (SL6241R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-TM4SF3 antibody (SL6241R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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