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Product Name TMED4 Chinese Name Transmembrane proteinTMED4抗体 Alias HNLF; Transmembrane emp24 domain containing protein 4; TMED4_HUMAN. Research Area Tumour Chromatin and nuclear signals Signal transduction transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 23kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TMED4: 41-140/227 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail TMED4 is a putative NF-kB activating protein belonging to the EMP24/GP25L family, and contains 1 GOLD domain. It is also known as Transmembrane emp24 protein transport domain containing 4.
Function:
Involved in vesicular protein trafficking, mainly in the early secretory pathway. targeting. Involved in the maintenance of the Golgi apparatus. Appears to play a role in the biosynthesis of secreted cargo including processing. Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis
Subcellular Location:
Endoplasmic reticulum membrane; Single-pass type I membrane protein
Similarity:
Belongs to the EMP24/GP25L family.
Contains 1 GOLD domain.
SWISS:
Q7Z7H5
Gene ID:
222068
Database links:Entrez Gene: 222068 Human
Entrez Gene: 103694 Mouse
Omim: 612038 Human
SwissProt: Q7Z7H5 Human
SwissProt: Q8R1V4 Mouse
Unigene: 598832 Human
Unigene: 715273 Human
Unigene: 254495 Mouse
Product Picture Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-TMED4 (SL6208R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMED4) Polyclonal Antibody, Unconjugated (SL6208R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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