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Product Name NCKAP1 Chinese Name 膜相关的蛋白质HEM2抗体 Alias NCKP1_HUMAN; Nck-associated protein 1; NAP 1; Membrane-associated protein HEM-2; p125Nap1; HEM2; KIAA0587; NAP1; NAP125; Research Area Tumour Cell biology Neurobiology Signal transduction Stem cells Apoptosis transcriptional regulatory factor The cell membrane蛋白 Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, Guinea Pig, ) Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 129kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NCKAP1: 1021-1128/1128 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Contributes to small GTPase binding activity. Involved in Rac protein signal transduction; positive regulation of Arp2/3 complex-mediated actin nucleation; and positive regulation of lamellipodium assembly. Located in extracellular exosome and focal adhesion. Part of SCAR complex. [provided by Alliance of Genome Resources, Apr 2022]
Function:
Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.
Subcellular Location:
Cell membrane. Cell projection
Tissue Specificity:
Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle. Expressed in cells of various brain regions including Purkinje cells and dentate nucleus of the cerebellum, CA4 region and dentate gyrus of the hippocampus, and in frontal gray and white matter
Similarity:
Belongs to the HEM-1/HEM-2 family.
SWISS:
Q9Y2A7
Gene ID:
10787
Database links:Entrez Gene: 10787 Human
Entrez Gene: 50884 Mouse
SwissProt: Q9Y2A7 Human
SwissProt: P28660 Mouse
Product Picture Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human 293T cell lysates
Primary: Anti-NCKAP1 (SL6204R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129 kDa
Observed band size: 135 kDa
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