Product Name	RASGEF1A
Chinese Name	鸟嘌呤核苷酸交换因子rasgef1a抗体
Alias	CG4853 gene product; Ras-GEF domain-containing family member 1A; RasGEF domain family, member 1A; rasgef1a; RGF1A_HUMAN; RASGEF 1A; RASGEF1A.
Research Area	Tumour  Signal transduction  Apoptosis  transcriptional regulatory factor  G protein-coupled receptor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Guinea Pig, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	53kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human RASGEF1A: 301-200/481
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Guanine nucleotide exchange factor (GEF) with specificity for RAP2A, KRAS, HRAS, and NRAS (in vitro). Plays a role in cell migration. Tissue Specificity: Detected in brain and spinal cord. Highly expressed in a number of intrahepatic cholangiocarcinoma tissue biopsies. Similarity: Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-GEF domain. SWISS: Q8N9B8 Gene ID: 221002 Database links: Entrez Gene: 221002 Human Entrez Gene: 70727 Mouse Entrez Gene: 312664 Rat SwissProt: Q8N9B8 Human Unigene: 125293 Human Unigene: 87660 Mouse Unigene: 29825 Rat
Product Picture	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-RASGEF1A Polyclonal Antibody, Unconjugated(SL6156R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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