Product Name	Wnt6
Chinese Name	信号通路Wnt6抗体
Alias	Protein Wnt-6 precursor; WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY, MEMBER 6; wnt 6; WNT6_HUMAN.
literatures	Specific References  (1)     |     SL6135R has been referenced in 1 publications. [IF=2.945] Zhang, Zilong. et al. Circ_FBLN1 promotes the proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by regulating let-7i-5p/FZD4 axis and Wnt/β-catenin pathway. 2021 Aug 23  WB ;  Human.   PubMed:34424449
Research Area	Tumour  Cell biology  immunology  Chromatin and nuclear signals  Cyclin  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	38kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Wnt6: 281-365/365
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Wnt6 is a member of the WNT protein family. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt6 is overexpressed in cervical and breast cell carcinomas and is co-expressed with Wnt10a in colorectal cancer cell lines. Subunit: Interacts with PORCN. Subcellular Location: Secreted, extracellular space, extracellular matrix. Similarity: Belongs to the Wnt family. SWISS: Q9Y6F9 Gene ID: 7475 Database links: Entrez Gene: 7475 Human Entrez Gene: 22420 Mouse Entrez Gene: 316526 Rat Omim: 604663 Human SwissProt: Q9Y6F9 Human SwissProt: P22727 Mouse Unigene: 29764 Human Unigene: 268282 Mouse
Product Picture	Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-Wnt6 (SL6135R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 48 kD Sample: MKN45(Human) Cell Lysate at 30 ug Primary: Anti-Wnt6 (SL6135R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 42 kD Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Wnt6 Polyclonal Antibody, Unconjugated(SL6135R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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