Product Name	WNT4
Chinese Name	信号通路Wnt4抗体
Alias	WNT4_HUMAN; Protein Wnt-4.
literatures	Specific References  (6)     |     SL6134R has been referenced in 6 publications. [IF=17.694] Katsumoto, Keiichi. et al. Wnt4 is heterogeneously activated in maturing β-cells to control calcium signaling, metabolism and function. NAT COMMUN. 2022 Oct;13(1):1-15  IHC ;  Mouse.   PubMed:36271049 [IF=5.16] Hua, Jun-yi, et al. "Emodin prevents intima thickness via Wnt4/Dvl-1/β-catenin signaling pathway mediated by miR-126 in balloon-injured carotid artery rats." Experimental & Molecular Medicine 47.6 (2015): e170.  IHC-P ;  Rat.   PubMed:26113441 [IF=2.829] Sung, Hsin-Ju Chiang, et al. "Combined therapy with melatonin and exendin-4 effectively attenuated the deterioration of renal function in rat cardiorenal syndrome." American Journal of Translational Research 9.2 (2017): 214-229.  IHC-P ;  Rat.   PubMed:28337255 [IF=2.69] Chang et al. Enhanced protection against renal ischemia-reperfusion injury with combined melatonin and exendin-4 in a rodent model. (2016) Exp.Biol.Med.(Maywood. 241:1588-602  IF(IHC-P) ;  Rat.   PubMed:27037275 [IF=1.257] Hai Zhao. et al. Oxidative stress caused by a dysregulated Wnt/β-catenin signalling pathway is involved in abnormal placenta formation in pregnant mice with chronic fatigue syndrome. Zygote. 2020 Oct;:1-8  WB,IHC ;  Mouse.   PubMed:33054899 [IF=0] Hua, Jun-yi, et al. "Wnt4/β-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1." Int J Clin Exp Pathol 7.12 (2014): 8421-8431.  IHC-P ;  Rat.   PubMed:25674206
Research Area	Tumour  Cell biology  immunology  Chromatin and nuclear signals  Cyclin  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	37kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human WNT4: 201-300/351
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue. Function: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue. Subunit: Interacts with PORCN. Subcellular Location: Secreted, extracellular space, extracellular matrix. DISEASE: Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome) [MIM:277000]; also called Mayer-Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls. Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL) [MIM:611812]; also known as SERKAL syndrome. Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL) [MIM:158330]. Similarity: Belongs to the Wnt family. SWISS: P56705 Gene ID: 54361 Database links: Entrez Gene: 54361 Human Entrez Gene: 22417 Mouse Omim: 603490 Human SwissProt: P56705 Human SwissProt: P22724 Mouse Unigene: 25766 Human Unigene: 611722 Human Unigene: 20355 Mouse
Product Picture	Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti- WNT4 (SL6134R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 47 kD Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug Lane 2: MCF-7 (Human) Cell Lysate at 30 ug Lane 3: A431 (Human) Cell Lysate at 30 ug Lane 4: U2os (Human) Cell Lysate at 30 ug Primary: Anti-WNT4 (SL6134R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 47 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT4) Polyclonal Antibody, Unconjugated (SL6134R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT4) Polyclonal Antibody, Unconjugated (SL6134R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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