Product Name	WNT2
Chinese Name	信号通路Wnt2抗体
Alias	Int 1 related protein; IRP protein; WNT 2; INT1L 1; INT1L1; ONCOGENE INT1 LIKE 1; Wingless type MMTV integration site family member 2; WNT2_HUMAN.
literatures	Specific References  (2)     |     SL6133R has been referenced in 2 publications. [IF=7.666] Bohao Zhao. et al. lncRNA2919 Suppresses Rabbit Dermal Papilla Cell Proliferation via trans-Regulatory Actions. CELLS-BASEL. 2022 Jan;11(15):2443  WB ;  Rabbit.   PubMed:35954286 [IF=3.71] Król, Magdalena, et al. "Macrophages Mediate a Switch between Canonical and Non-Canonical Wnt Pathways in Canine Mammary Tumors." PloS one 9.1 (2014): e83995  WB ;  Dog.   PubMed:24404146
Research Area	Tumour  Cell biology  Chromatin and nuclear signals  Signal transduction  Cyclin  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,
Applications	WB=1:500-2000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	37kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human WNT2: 221-320/360
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Subcellular Location: Secreted. Tissue Specificity: Expressed in brain in the thalamus, in fetal and adult lung and in placenta. Similarity: Belongs to the Wnt family. SWISS: P09544 Gene ID: 7472 Database links: Entrez Gene: 7472 Human Entrez Gene: 22413 Mouse Entrez Gene: 114487 Rat Omim: 147870 Human SwissProt: P09544 Human SwissProt: P21552 Mouse Unigene: 567356 Human Unigene: 33653 Mouse
Product Picture	Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti- WNT2 (SL6133R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 37 kD Observed band size: 38 kD Sample: Lane 1: Human HeLa cell lysates Lane 2: Human MCF-7 cell lysates Lane 3: Human Jurkat cell lysates Primary: Anti-WNT2 (SL6133R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 35 kDa Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain tissue with Parkinson's morphology labeled with Rabbit Anti-WNT2 Polyclonal Antibody (SL6133R) at 1:250 overnight at 4 °C followed by conjugation to secondary antibody. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT2) Polyclonal Antibody, Unconjugated (SL6133R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain glioma tissue labeled with Rabbit Anti-WNT2 Polyclonal Antibody (SL6133R) at 1:250 overnight at 4 °C followed by conjugation to secondary antibody.

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