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Product Name SHPRH Chinese Name 组蛋白连接作用蛋白抗体 Alias E3 ubiquitin-protein ligase SHPRH; histone-linker; PHD and RING finger domain-containing helicase; SNF2; SNF2 histone linker PHD RING helicase; SHPRH_HUMAN. Research Area Tumour Cell biology Cyclin Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 193kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SHPRH: 401-500/1683 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5
Function:
E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.
Subunit:
Homodimer. Interacts with HLTF, PCNA, UBE2N and RAD18.
Tissue Specificity:
Broadly expressed.
Similarity:
Belongs to the SNF2/RAD54 helicase family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger.
SWISS:
Q149N8
Gene ID:
257218
Database links:Entrez Gene: 257218 Human
Omim: 608048 Human
SwissProt: Q149N8 Human
Unigene: 723297 Human
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