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Product Name RND3 Chinese Name Rho家族GTP酶3抗体 Alias ARHE; memB; Rho family GTPase 3; Rho8; RhoE; RND3_HUMAN. Research Area Tumour Cell biology Chromatin and nuclear signals Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 27kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human RND3: 51-150/244 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The Rho family of proteins mediates cytoskeleton organization and cell motility responses. This family contains twenty different members divided into five groups, the Rho-like, Rac-like, Cdc42-like, Rnd and RhoBTB subfamilies. Rho proteins have GTPase activity and bind GTP in their active form and GDP in their inactive form. In their active form they interact with downstream proteins. However, the Rnd subfamily of proteins containing the Rnd1, Rnd2 and RhoE(Rnd3), binds GTP but not GDP and has no GTPase activity. While Rho proteins are required for stress fibre formation in cultured fibroblasts, epithelial and endothelial cells, overexpression of Rnd3 or Rnd1 in the same cells may cause an opposite effect (i.e. decrease in stress fibre). Thus, the Rnd3 protein may act as an antagonist for the Rho proteins either by directly binding to Rho or to other downstream proteins.
Subunit:
Binds ROCK1 (By similarity). Interacts with UBXD5.
Subcellular Location:
Golgi Apparatus; Golgi apparatus membrane; peripheral membrane protein.
Tissue Specificity:
Ubiquitous.
Similarity:
Belongs to the small GTPase superfamily. Rho family.
SWISS:
P61587
Gene ID:
390
Database links:Entrez Gene: 390 Human
Entrez Gene: 74194 Mouse
Omim: 602924 Human
SwissProt: P61587 Human
SwissProt: P61588 Mouse
Unigene: 6838 Human
Unigene: 46497 Mouse
Unigene: 25153 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RND3) Polyclonal Antibody, Unconjugated (SL6095R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RND3) Polyclonal Antibody, Unconjugated (SL6095R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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