TEL: +86 571 56623320 EMAIL: [email protected]
Product Name CASC5 Chinese Name Tumour易感性候选基因5抗体 Alias Protein D40/AF15q14; AF15Q14; ALL1 fused gene from chromosome 15q14; Blinkin bub linking kinetochore protein; Cancer susceptibility candidate 5; Cancer susceptibility candidate gene 5 protein; Cancer/testis antigen 29; CT29; D40; hKNL 1; hSpc105; KIAA1570; Kinetochore null 1 homolog; Kinetochore null protein 1; KNL1; CASC5_HUMAN. literatures Specific References (1) | SL5959R has been referenced in 1 publications.[IF=0] Lu, Zhimin, and Alfred WK Yung. "BIOMARKERS FOR CANCER CHARACTERIZATION AND TREATMENT." U.S. Patent No. 20,150,073,033. 12 Mar. 2015. IP ;Research Area Tumour Cell biology immunology Stem cells Cyclin Cell differentiation Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 265kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CASC5: 2251-2342/2342 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Four known isoforms exist.
Function:
Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.
Subunit:
Interacts with DSN1, MIS12, BUB1, BUB1B, NSL1 and ZWINT.
Subcellular Location:
Nucleus. Chromosome, centromere, kinetochore.
Tissue Specificity:
Highly expressed in testis, where it is localized in germ cells, in particular in spermatocytes and in the pre-acrosome of round spermatids. Detected in the acrosome of ejaculated spermatozoa. Detected in adult thymus, bone marrow, colon, small intestine, appendix and placenta, and in fetal liver and thymus.
DISEASE:
Note=A chromosomal aberration involving CASC5 is associated with acute myeloblastic leukemia (AML). Translocation t(11;15)(q23;q14) with MLL/HRX. May give rise to a MLL-CASC5 fusion protein.
SWISS:
Q8NG31
Gene ID:
57082
Database links:Entrez Gene: 57082 Human
Entrez Gene: 76464 Mouse
Omim: 609173 Human
SwissProt: Q8NG31 Human
SwissProt: Q66JQ7 Mouse
Product Picture Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti- CASC5 (SL5959R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 265 kD
Observed band size: 265 kD
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CASC5) Polyclonal Antibody, Unconjugated (SL5959R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Scan Wechat Qrcode