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Product Name PBOV1 Chinese Name 前列腺癌和乳腺癌高表达蛋白抗体 Alias PBOV 1; PBOV-1; Prostate and breast cancer overexpressed gene 1 protein; UROC28; Prostate and breast cancer overexpressed 1; UC28.PBOV1_HUMAN Research Area Tumour immunology Chromatin and nuclear signals Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 15kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PBOV1: 31-130/135 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene is overexpressed in prostate and breast cancer.
Subcellular Location:
Cytoplasm. Nucleus.
Tissue Specificity:
Expressed in colon, prostate, small intestine, testis and spleen, with lower expression in thymus, ovary, and peripheral blood leukocytes. Up-regulated expression in prostate, breast, and bladder cancer, but not in lung and colon cancer.
SWISS:
Q9GZY1
Gene ID:
59351
Database links:Entrez Gene: 59351 Human
Omim: 605669 Human
SwissProt: Q9GZY1 Human
Unigene: 302016 Human
PBOV1蛋白,主要表达在前列腺癌和乳腺癌等。Product Picture Tissue/cell: human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PBOV1 Polyclonal Antibody, Unconjugated(SL5939R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PBOV1) Polyclonal Antibody, Unconjugated (SL5939R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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