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Product Name GADD45B Chinese Name 生长抑制DNA损伤基因GADD45β抗体 Alias GA45B_HUMAN; GADD45 Beta; Gadd45b; GADD45BETA; Growth arrest and DNA damage inducible 45 beta; Growth arrest and DNA damage inducible beta; Growth arrest and DNA damage-inducible protein GADD45 beta; Growth arrest and DNA-damage-inducible protein GADD45 beta; MYD118; Myeloid differentiation primary response gene; Myeloid differentiation primary response protein MyD118; Negative growth regulatory protein MyD118; Negative growth-regulatory protein MyD118. Research Area Tumour Cell biology immunology Signal transduction Apoptosis Growth factors and hormones Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, Guinea Pig, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 18kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GADD45B.: 81-160/160 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.
Function:
Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.
Subunit:
Interacts with GADD45GIP1.
Similarity:
Belongs to the GADD45 family.
SWISS:
O75293
Gene ID:
4616
Database links:
Entrez Gene: 4616 Human
Entrez Gene: 17873 Mouse
Omim: 604948 Human
SwissProt: O75293 Human
SwissProt: P22339 Mouse
GADD45β; GADD45 β; GADD45-β.Product Picture Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Rat Liver tissue lysates
Lane 3: Rat Kidney tissue lysates
Primary: Anti-GADD45B (SL5904R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GADD45B) Polyclonal Antibody, Unconjugated (SL5904R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GADD45B Polyclonal Antibody, Unconjugated(SL5904R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GADD45B Polyclonal Antibody, Unconjugated(SL5904R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-GADD45B antibody (SL5904R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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