TEL: +86 571 56623320 EMAIL: [email protected]
Product Name PPP2R3 Chinese Name 蛋白质磷酸酶2A亚基3抗体 Alias AI118493; DNA for thyroid hormone receptor binding site (258bp); P2R3A_HUMAN; PP2A subunit B B72/B130 isoforms; PP2A subunit B isoform PR72/PR130; PP2A subunit B isoform R3 isoform; PP2A subunit B isoforms B72/B130; PP2A subunit B PR72/PR130 isoforms; PP2A subunit B R3 isoform; Ppp2r3a; PR130; PR59; PR72; Serine/threonine protein phosphatase 2A 72/130 kDa regulatory subunit B; Serine/threonine-protein phosphatase 2A 72/130 kDa regulatory subunit B. Research Area Tumour Cell biology immunology Signal transduction Cyclin Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 130kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PPP2R3: 801-900/1150 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Function:
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Subunit:
PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
Tissue Specificity:
Expressed in heart, brain, placenta, lung, muscle and kidney.
Similarity:
Contains 2 EF-hand domains.
SWISS:
Q06190
Gene ID:
5523
Database links:Entrez Gene: 5523 Human
Omim: 604944 Human
SwissProt: Q06190 Human
Unigene: 518155 Human
Product Picture Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti-PPP2R3 (SL5759R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti- PPP2R3 (SL5759R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
Sample:
Skeletal muscle (Mouse) Lysate at 40 ug
Primary: Anti- PPP2R3 (SL5759R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP2R3) Polyclonal Antibody, Unconjugated (SL5759R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Scan Wechat Qrcode