Rabbit Anti-DBF4B antibody
Activator of S phase kinase like protein 1; ASK like protein 1; ASKL1; chifb; Chiffon homolog B; DBF 4B; DBF4 homolog B (S. cerevisiae); DBF4 homolog B; Dbf4 related factor 1; DRF1; Protein DBF4 homolog B; ZDBF1B; Zinc finger DBF type containing 1B; DBF4B
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Product Name DBF4B Chinese Name S期激酶活化蛋白DBF4B抗体 Alias Activator of S phase kinase like protein 1; ASK like protein 1; ASKL1; chifb; Chiffon homolog B; DBF 4B; DBF4 homolog B (S. cerevisiae); DBF4 homolog B; Dbf4 related factor 1; DRF1; Protein DBF4 homolog B; ZDBF1B; Zinc finger DBF type containing 1B; DBF4B_HUMAN. Research Area Tumour Cell biology immunology Signal transduction Cyclin Kinases and Phosphatases Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 18kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human DBF4B/ASKL1: 41-140/615 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail DBF4B is a regulatory subunit for CDC7, a serine-threonine kinase which links cell cycle regulation to genome duplication. The complex CDC7-DBF4B selectively phosphorylates the MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. DBF4B localizes to the nucleus and its expression is cell cycle-regulated.
Function:
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.
Subunit:
Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4/DBF4A or DBF4B. Such complexes are stable upon replication stress.
Subcellular Location:
Nucleus. Note=Predominantly found in soluble fraction but not in the chromatin-bound fraction.
Tissue Specificity:
Widely expressed. Highly expressed in testis.
Post-translational modifications:
Phosphorylated.
Similarity:
Contains 1 BRCT domain.
Contains 1 DBF4-type zinc finger.
SWISS:
Q8NFT6
Gene ID:
80174
Database links:Entrez Gene: 80174 Human
Omim: 611661 Human
SwissProt: Q8NFT6 Human
Unigene: 369998 Human
Unigene: 728986 Human
Product Picture 
Sample: Hela Cell (Human) Lysate at 30 ug
Primary: Anti-DBF4B (SL5748R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18kD
Observed band size: 18kD
Sample: 293T Cell (Human) Lysate at 30 ug
Primary: Anti-DBF4B (SL5748R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18kD
Observed band size: 18kD
Sample: U2os Cell (Human) Lysate at 30 ug
Primary: Anti-DBF4B (SL5748R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18kD
Observed band size: 18kD
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