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Product Name CDKN3 Chinese Name Cyclin依赖性激酶抑制蛋白3抗体 Alias Cdk associated protein phosphatase; CDI1; CDK2 associated dual specificity phosphatase; CIP2; Cyclin dependent kinase inhibitor 3; Cyclin dependent kinase interactor 1; Cyclin dependent kinase-interacting protein 2; KAP1; Kinase associated phosphatase. literatures Specific References (1) | SL5743R has been referenced in 1 publications.[IF=12.822] Sha Li. et al. Identification and validation of functional roles for three MYC-associated genes in hepatocellular carcinoma. J ADV RES. 2023 Jan;: IHC ; Mouse.Research Area Tumour Cell biology immunology Cyclin Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 24kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CDKN3: 51-150/212 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail CDKN3 is a second dual specificity phosphatase that interacts with cyclin dependent kinases.The ability of CDKN3 to bind multiple cyclin-dependent kinases suggests that it may be a critical control element in cell cycle regulation, presumably by regulating the phosphorylation state of a Cdk or Cdk-associated protein.
Function:
May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner.
Subunit:
Interacts with cyclin-dependent kinases such as CDK1, CDK2 and CDK3. Does not interact with CDK4. Interacts (via C-terminus) with phosphorylated CDK2 (via C-terminal helix). Interacts with MS4A3 (via C-terminus); the interaction enhances CDKN3 enzymatic activity.
Subcellular Location:
Cytoplasm, perinuclear region.
DISEASE:
Defects in CDKN3 are found in patients with hepatocellular carcinoma (HCC) [MIM:114550].
Similarity:
Belongs to the protein-tyrosine phosphatase family.
SWISS:
Q16667
Gene ID:
1033
Database links:Entrez Gene: 1033 Human
Entrez Gene: 72391 Mouse
Omim: 123832 Human
SwissProt: Q16667 Human
SwissProt: Q810P3 Mouse
Unigene: 84113 Human
Unigene: 272394 Mouse
Unigene: 107220 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (human Prostate Tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDKN3) Polyclonal Antibody, Unconjugated (SL5743R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDKN3) Polyclonal Antibody, Unconjugated (SL5743R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDKN3) Polyclonal Antibody, Unconjugated (SL5743R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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