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Product Name phospho-STAT5b (Ser731) Chinese Name 磷酸化Signal transduction和转录激活因子5b抗体 Alias STAT5b (phospho S731); p-STAT5b (phospho S731); STAT5b(phospho S731); Transcription factor STAT5B; Signal transducer and activator of transcription 5B; STA5B_HUMAN; STAT5; Stat5b. literatures Specific References (2) | SL5703R has been referenced in 2 publications.[IF=3.738] Zong, Jinxin. et al. Lithium Chloride Promotes Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells via HIF-1α and β-Catenin Signaling Pathways. Biol Trace Elem Res. 2022 Jan;:1-16 WB ; Bovine.[IF=1.227] Liao XD et al. Effect of all-trans retinoic acid on casein and fatty acid synthesis in MAC-T cells. Asian-Australas J Anim Sci. 2019 Aug 23. WB ; bovine.Product Type Phosphorylated anti Research Area Tumour Cell biology immunology Chromatin and nuclear signals Signal transduction transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 90kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human STAT5b around the phosphorylation site of Ser731: AP(p-S)PA Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Function:
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.
Subunit:
Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1, NMI and SOCS7. Interacts (via SH2 domain) with INSR.
Subcellular Location:
Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocated into the nucleus in response to phosphorylation (By similarity).
Post-translational modifications:
Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphoryation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity.
DISEASE:
Growth hormone insensitivity with immunodeficiency (GHII) [MIM:245590]: A disease characterized by short stature, growth hormone deficiency in the presence of normal to elevated circulating concentrations of growth hormone, resistance to hexogeneous growth hormone therapy, and recurrent infections. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity:
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
SWISS:
P51692
Gene ID:
6777
Database links:Entrez Gene: 6777 Human
Entrez Gene: 20851 Mouse
Omim: 604260 Human
SwissProt: P51692 Human
SwissProt: P42232 Mouse
Unigene: 595276 Human
Unigene: 34064 Mouse
Unigene: 54486 Rat
Product Picture Blank control (Black line):Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-phospho-STAT5b (Ser731) antibody (SL5703R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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