Product Name	phospho-TPH (Ser260)
Chinese Name	磷酸化色氨酸羟化酶抗体
Alias	Tph1(phospho S260); TPH (phospho Ser260); p-TPH (Ser260); MGC119994; TPH 1; TPH; TPH1; TPRH; Tryptophan 5 hydroxylase 1; Tryptophan 5 monooxygenase 1; Tryptophan 5 monooxygenase; Tryptophan hydroxylase 1; Tryptophan 5-hydroxylase 1; TPH1_HUMAN.
Product Type	Phosphorylated anti
Research Area	immunology  Neurobiology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	49kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human TPH around the phosphorylation site of Ser260: HS(p-S)DP
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009]. Subunit: Homotetramer (By similarity). Tissue Specificity: Isoform 2 seems to be less widely expressed than isoform 1. Similarity: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain. SWISS: P17752 Gene ID: 7166 Database links: Entrez Gene: 7166 Human Entrez Gene: 21990 Mouse Entrez Gene: 24848 Rat Omim: 191060 Human SwissProt: P17752 Human SwissProt: P17532 Mouse SwissProt: P09810 Rat Unigene: 591999 Human Unigene: 248684 Mouse Unigene: 94788 Rat 色氨酸在脑中首先由色氨酸羟化酶(tryoptophan hydroxylase)催化生成5羟色氨酸(5hydroxytryptophan)，再经脱羧酶作用生成5羟色胺，色氨酸羟化酶（TPH）是5-HT生物合成的限速酶，因此，色氨酸羟化酶的缺少可导致5-羟色胺的含量下降。色氨酸羟化酶主要在肝脏内代谢。
Product Picture	Sample: Cerebrum(Rat) Lysate at 40 ug Primary: Anti-phospho-TPH (Ser260) (SL5601R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD Sample: HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-phospho-TPH (Ser260) (SL5601R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 49 kD Observed band size: 49 kD Sample: Lane 1: Mouse Cerebellum tissue lysates Lane 2: Rat Cerebellum tissue lysates Primary: Anti-phospho-TPH (Ser260) (SL5601R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 50 kDa Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-phospho-TPH (Ser260) (SL5601R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TPH (Ser260)) Polyclonal Antibody, Unconjugated (SL5601R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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