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Rabbit Anti-phospho-14-3-3 epsilon (Thr232)antibody
Rabbit Anti-phospho-14-3-3 epsilon (Thr232)antibody
YWHAE(phospho T232); 14-3-3 epsilon (phospho Thr232); 14-3-3 epsilon; 14 3 3 E; 14 3 3 epsilon; 14 3 3E; 14-3-3 E; 14-3-3 protein epsilon; 14-3-3E; 14-3-3ε; 1433E_HUMAN; KCIP 1; KCIP-1; KCIP1; MDCR; MDS; mitochondrial import stimulation factor L subunit;
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  • NO.:SL5590R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Human,(predicted: Mouse,Rat,Cow,Sheep,)
    Applications:ELISA Flow-Cyt
    concentration:1mg/ml
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Product Name phospho-14-3-3 epsilon (Thr232)
Chinese Name 磷酸化14-3-3E蛋白抗体
Alias YWHAE(phospho T232); 14-3-3 epsilon (phospho Thr232); 14-3-3 epsilon; 14 3 3 E; 14 3 3 epsilon; 14 3 3E; 14-3-3 E; 14-3-3 protein epsilon; 14-3-3E; 14-3-3ε; 1433E_HUMAN; KCIP 1; KCIP-1; KCIP1; MDCR; MDS; mitochondrial import stimulation factor L subunit; protein kinase C inhibitor protein-1; Protein kinase C inhibitor protein1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein epsilon polypeptide; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide; YWHAE.  
Product Type Phosphorylated anti 
Research Area immunology  Neurobiology  Signal transduction  Stem cells  Apoptosis  Cell type markers  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Human,  (predicted: Mouse, Rat, Cow, Sheep, )
Applications ELISA=1:5000-10000 Flow-Cyt=1μg/Test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 29kDa
Cellular localization The nucleus 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated Synthesised phosphopeptide derived from human 14-3-3E around the phosphorylation site of Thr232: LW(p-T)SD 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Function:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Subunit:
Homodimer. Heterodimerizes with YWHAZ. Interacts with NDEL1, RGNEF and TIAM2. Interacts with HCV core protein. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B. Interacts with GAB2. Interacts with phosphorylated GRB10. Interacts with PKA-phosphorylated AANAT. Interacts with the phosphorylated (by AKT1) form of SRPK2.

Subcellular Location:
Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Similarity:
Belongs to the 14-3-3 family.

SWISS:
P62258

Gene ID:
7531

Database links:

Entrez Gene: 7531 Human

Entrez Gene: 282125 Cow

Entrez Gene: 22627 Mouse

Entrez Gene: 29753 Rat

Omim: 605066 Human

SwissProt: P62261 Cow

SwissProt: P62258 Human

SwissProt: P62259 Mouse

SwissProt: P62260 Rat

Unigene: 513851 Human

Unigene: 234700 Mouse

Unigene: 471625 Mouse

Unigene: 4225 Rat



Product Picture
Blank control (blue line): MCF7,fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice
Primary Antibody (green line): Rabbit Anti-phospho-14-3-3 epsilon (Thr232) antibody (SL5590R),Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

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