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Product Name phospho-PPM1A (Ser375) Chinese Name 磷酸化蛋白磷酸酶2C亚型α抗体 Alias PPM1A(phospho Ser375); PPM1A(phospho S375); Mpp alpha; PP2C alpha; PP2C-alpha; PP2CA; PPM 1A; PPM1A; PPM1A_HUMAN; PPPM1A; Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform; Protein phosphatase 1A; Protein phosphatase 1A magnesium dependent alpha; Protein phosphatase 2C alpha; Protein phosphatase 2C alpha isoform; Protein phosphatase 2C isoform alpha; Protein phosphatase IA; Protein phosphatase 2C isoform alpha; IA antibody. Product Type Phosphorylated anti Research Area immunology Chromatin and nuclear signals Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Dog, Cow, Sheep, Guinea Pig, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 42kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human PPM1A around the phosphorylation site of Ser375: TD(p-S)TS Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008].
Function:
Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling.
Subunit:
Monomer. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the PP2C family.
SWISS:
P35813
Gene ID:
5494
Database links:Entrez Gene: 5494 Human
Entrez Gene: 19042 Mouse
Omim: 606108 Human
SwissProt: P35813 Human
SwissProt: P49443 Mouse
Unigene: 130036 Human
Unigene: 261045 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PPM1A(Ser375)) Polyclonal Antibody, Unconjugated (SL5586R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PPM1A(Ser375)) Polyclonal Antibody, Unconjugated (SL5586R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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