Product Name	phospho-MYL6 (Tyr29+Ser30)
Chinese Name	磷酸化肌球蛋白轻链6抗体
Alias	MYL6 (phospho-Tyr29+Ser30); MYL6 (phospho Y29+S30); ESMLC; LC 17; LC17; LC17 GI; LC17 NM; LC17A; LC17B; MLC 3; MLC-3; MLC1SM; MLC3; MLC3NM; MLC3SM; MYL 6; MYL6; MYL6_HUMAN; Myosin light chain 3; Myosin light chain 6 alkali smooth muscle and non muscle; Myosin light chain A3; Myosin light chain alkali 3; Myosin light polypeptide 6 alkali smooth muscle and non muscle; Myosin light polypeptide 6; Smooth muscle and non muscle myosin alkali light chain; Smooth muscle and nonmuscle myosin light chain alkali 6; 17 kDa myosin light chain.
Product Type	Phosphorylated anti
Research Area	Cardiovascular  immunology  Signal transduction  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Chicken, Pig, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	17kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human MYL6 around the phosphorylation site of Tyr29+Ser30: IL(p-Y)(p-S)Q-NH2
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene. Function: Regulatory light chain of myosin. Does not bind calcium. Subunit: Myosin is a hexamer of 2 heavy chains and 4 light chains. Subcellular Location: Cytoplasm. Nucleus. Similarity: Contains 3 EF-hand domains. SWISS: P60660 Gene ID: 4637 Database links: Entrez Gene: 4637 Human Entrez Gene: 17904 Mouse Entrez Gene: 396807 Pig Entrez Gene: 362816 Rat Entrez Gene: 685867 Rat Omim: 609931 Human SwissProt: P60661 Cow SwissProt: P60660 Human SwissProt: Q60605 Mouse SwissProt: P60662 Pig SwissProt: Q64119 Rat Unigene: 632717 Human Unigene: 337074 Mouse Unigene: 146093 Rat
Product Picture	Tissue/cell: muscle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-MYL6(Tyr29+Ser30) Polyclonal Antibody, Unconjugated(SL5478R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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