Product Name	phospho-LRP1 (Ser4523)
Chinese Name	磷酸化低密度Lipoprotein受体相关蛋白1抗体
Alias	LRP1 (phospho Ser4523); LRP1 (phospho S4523); A2MR; Alpha 2 macroglobulin receptor; alpha 2MR; APOER; Apolipoprotein E receptor; APR; CD 91; CD91; CD91 antigen; Low density lipoprotein receptor related protein 1; Low density lipoprotein related protein 1; LRP 1; LRP 515; LRP 85; LRP; LRP ICD; LRP1 protein; LRP515; LRP1_HUMAN.
Product Type	Phosphorylated anti
Research Area	Cardiovascular  immunology  Neurobiology  Signal transduction  Synthesis and Degradation
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	85kDa/515kDa
Cellular localization	The nucleus cytoplasmic The cell membrane Extracellular matrix
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human LRP1 around the phosphorylation site of Ser4523: LA(p-S)TD <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the low-density lipoprotein receptor family of proteins. The encoded preproprotein is proteolytically processed by furin to generate 515 kDa and 85 kDa subunits that form the mature receptor (PMID: 8546712). This receptor is involved in several cellular processes, including intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. In addition, the encoded protein is necessary for the alpha 2-macroglobulin-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer's disease patients. [provided by RefSeq, Oct 2015] Subunit: Heterodimer of an 85-kDa membrane-bound carboxyl subunit and a non-covalently attached 515-kDa N-terminal subunit. Intracellular domain interacts with MAFB (By similarity). Found in a complex with PID1/PCLI1, LRP1 and CUBNI. Interacts with SNX17, PID1/PCLI1, PDGF and CUBN. The intracellular domain interacts with SHC1, GULP1 and DAB1. Interacts with LRPAP1. Interacts with bacterial exotoxins. Subcellular Location: Low-density lipoprotein receptor-related protein 1 85 kDa subunit: Cell membrane; Single-pass type I membrane protein. Membrane, coated pit. Low-density lipoprotein receptor-related protein 1 515 kDa subunit: Cell membrane; Peripheral membrane protein; Extracellular side. Membrane, coated pit. Low-density lipoprotein receptor-related protein 1 intracellular domain: Cytoplasm. Nucleus. Note=After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus. Tissue Specificity: Most abundant in liver, brain and lung. Similarity: Belongs to the LDLR family. Contains 22 EGF-like domains. Contains 31 LDL-receptor class A domains. Contains 34 LDL-receptor class B repeats. SWISS: Q07954 Gene ID: 4035 Database links: Entrez Gene: 4035 Human Entrez Gene: 16971 Mouse Entrez Gene: 299858 Rat SwissProt: Q07954 Human SwissProt: Q91ZX7 Mouse
Product Picture	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-LRP1(Ser4523) Polyclonal Antibody, Unconjugated(SL5409R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control（black line）:HepG2. Primary Antibody (green line): Rabbit Anti-phospho-LRP1 (Ser4523) antibody (SL5409R) Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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