Product Name	Phospho-RhoA (Ser188)
Chinese Name	磷酸化RhoA蛋白抗体
Alias	RHOA(phospho S188); RhoA (phospho S188); RhoA protein; Aplysia ras related homolog 12; ARH 12; ARH12; ARHA; H 12; Oncogene RHO H12; Ras homolog gene family member A; RHO 12; Rho A; RHO H12; RHO12; RHOH 12; RHOH12; Small GTP binding protein Rho A; Small GTP binding protein RhoA; Transforming protein Rho A; Transforming protein RhoA.
literatures	Specific References  (5)     |     SL5330R has been referenced in 5 publications. [IF=5.82] Yijie Yang. et al. A Collagen-Derived Oligopeptide from Salmo salar Collagen Hydrolysates Restrains Atherogenesis in ApoE–/– Mice via Targeting P2Y12 Receptor. MOL NUTR FOOD RES. 2022 May 08  WB ;  Rat.   PubMed:35490399 [IF=5.279] Yijie Yang. et al. Novel Peptide Motifs Containing Asp-Glu-Gly Target P2Y12 and Thromboxane A2 Receptors to Inhibit Platelet Aggregation and Thrombus Formation. J Agr Food Chem. 2022;XXXX(XXX):XXX-XXX  WB ;  Rat.   PubMed:35016500 [IF=5.168] Gu et al. Fasudil attenuates soluble fms-like tyrosine kinase-1 (sFlt-1)-induced hypertension in pregnant mice through RhoA/ROCK pathway. (2017) Oncotarget. 8:104104-104112  WB, IHC-P ;  Human, Mouse.   PubMed:29262624 [IF=4.803] Yi Liu aet al. Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway. Genes & Diseases  WB ;  Human.   PubMed:10.1016/j.gendis.2020.07.009 [IF=2.38] Zhang, Beiru, et al. "HSF1 Relieves Amyloid-β-Induced Cardiomyocytes Apoptosis." Cell Biochemistry and Biophysics (2015): 1-9.  WB ;  Rat.   PubMed:25631374
Product Type	Phosphorylated anti
Research Area	Tumour  Signal transduction  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Chicken, Dog, Cow, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	21kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human RhoA around the phosphorylation site of Ser188: KK(p-S)GC
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. Subunit: nteracts with RGNEF. Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts (in the constitutively activated, GTP-bound form) with DGKQ. Interacts with human respiratory syncytial virus (HRSV) protein F; this interaction facilitates virus-induced syncytium formation. Interacts with GNB2L1/RACK1; enhances RHOA activation. Interacts with PKP4; the interaction is detected at the midbody. Interacts (GTP-bound form preferentially) with PKN2; the interaction stimulates autophosphorylation and phosphorylation of PKN2. Similarity: Belongs to the small GTPase superfamily. Rho family. SWISS: P61586 Gene ID: 387 Database links: Entrez Gene: 387 Human Entrez Gene: 11848 Mouse Entrez Gene: 117273 Rat Omim: 165390 Human SwissProt: P61586 Human SwissProt: Q9QUI0 Mouse SwissProt: P61589 Rat Unigene: 247077 Human Unigene: 757 Mouse Unigene: 107401 Rat RhoA蛋白主要分布在细胞质和The cell membrane,RhoA蛋白家族是控制Cytoskeleton蛋白组合、调节基因表达等细胞活动过程中的关键Signal transduction成分。通常,RhoA通过激活其下游的Rho 连接激酶(Rho associated kinase,ROK)而起作用。在调节Cytoskeleton组建的过程中,RhoA与其作用对象均在胞质内。
Product Picture	Sample: Brain (Rat) Lysate at 40 ug Primary: Anti-Phospho-RhoA(Ser188) (SL5330R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 21 kD Observed band size: 21 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-RhoA(Ser188)) Polyclonal Antibody, Unconjugated (SL5330R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Phospho-RhoA Antibody(SL5330R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). Blank control (Black line): Molt4 (Black). Primary Antibody (green line):Rabbit Anti-Phospho-RhoA (Ser188) antibody (SL 5330R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control (Black line): Molt4 (Black). Primary Antibody (green line):Rabbit Anti-Phospho-RhoA (Ser188) antibody (SL 5330R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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