Product Name	phospho-CD244 (Tyr271)
Chinese Name	磷酸化CD244抗体
Alias	CD244(phospho Y271); CD244(phospho-Tyr271); NKR2B4; SLAMF4; natural killer cell receptor 2B4 isoform 2 precursor; 2B4 ; C9.1; CD244 antigen; CD244 molecule natural killer cell receptor 2B4; CD244 natural killer cell receptor 2B4; F730046O15Rik; h2B4; Ly90; NAIL; Natural killer cell activation-inducing ligand; Natural killer cell receptor 2B4; NK cell activation inducing ligand; NK cell activation inducing ligand NAIL; NK cell type I receptor protein 2B4; Nmrk; Non-MHC restricted killing associated; OTTHUMP00000027884; p38; SLAM family, member 4.
literatures	Specific References  (1)     |     SL5284R has been referenced in 1 publications. [IF=4.047] Xu Xiaoyan. et al. A novel 2B4 receptor leads to worse pregnancy outcomes by facilitating TNF-α and IFN-γ production in dNK cells during Toxoplasma gondii infection. PARASITE VECTOR. 2022 Dec;15(1):1-15  WB ;  Human.   PubMed:36153598
Product Type	Phosphorylated anti
Research Area	Tumour  immunology  Signal transduction  Apoptosis  Cell Surface Molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Pig, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	38kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human CD244 around the phosphorylation site of Tyr271: TI(p-Y)ED
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	CD244 is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. CD244 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness. Although there have been several molecules shown to interact with CD244, the signaling mechanism ofCD244-mediated activation of NK cells is still unknown. It is a high affinity receptor for CD48. Function: Modulate other receptor-ligand interactions to enhance leukocyte activation. Subunit: Interacts with CD48. Following phosphorylation, it is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. Binding of SH2D1A/SAP to CD244 prevents its association with PTPN11/SHP-2. Subcellular Location: Membrane; Single-pass type I membrane protein (Potential). Tissue Specificity: Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils. Similarity: Contains 2 Ig-like (immunoglobulin-like) domains. SWISS: Q9BZW8 Gene ID: 51744 Database links: Entrez Gene: 51744 Human Entrez Gene: 18106 Mouse Entrez Gene: 64025 Rat Omim: 605554 Human SwissProt: Q9BZW8 Human SwissProt: Q07763 Mouse SwissProt: Q9JLM2 Rat Unigene: 157872 Human Unigene: 2299 Mouse Unigene: 113760 Rat Unigene: 53994 Rat
Product Picture	Sample: Hela (Human) Lysate at 40 ug Primary: Anti- phospho-CD244(Tyr271) (SL5284R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 65 kD Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-phospho-CD244(Tyr271) (SL5284R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 70 kD Blank control: U937. Primary Antibody (green line): Rabbit Anti-CD244 antibody (SL5284R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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