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Product Name MYLPF Chinese Name 快速骨骼肌肌球蛋白轻链2抗体 Alias 2410014J02Rik; DKFZp779C0757; DTNB; Fast skeletal myosin light chain 2; G2; HUMMLC2B; MGC13450; MLC 2; MRLC2; MYL11; MLC2B; MLC2F; MLRS_HUMAN; MYLPF; Myosin light polypeptide 2 alkali; Myosin regulatory light chain 2, skeletal muscle isoform. Research Area immunology Signal transduction Cytoskeleton Extracellular matrix Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 19kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Fast skeletal myosin light chain 2: 101-170/170 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of Myosin have been cloned: smooth muscle Myosins, striated muscle Myosins and non-muscle Myosins . Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle.
Subunit:
Myosin is a hexamer of 2 heavy chains and 4 light chains.
Tissue Specificity:
Expressed in fetal and adult skeletal muscle.
Similarity:
Contains 3 EF-hand domains.
SWISS:
Q96A32
Gene ID:
29895
Database links:Entrez Gene: 29895 Human
Entrez Gene: 396470 Chicken
Entrez Gene: 4632 Human
Entrez Gene: 17901 Mouse
Entrez Gene: 17907 Mouse
Omim: 160780 Human
SwissProt: P02609 Chicken
SwissProt: P05976 Human
SwissProt: P06741 Human
SwissProt: Q14324 Human
SwissProt: P05978 Mouse
SwissProt: P97457 Mouse
SwissProt: P02602 Rabbit
SwissProt: P02603 Rabbit
Product Picture Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Rat Muscle tissue lysates
Primary: Anti-MYLPF (SL5159R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kDa
Observed band size: 15 kDa
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-MYLPF (SL5159R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 19 kD
Sample:
A673(Human) Cell Lysate at 30 ug
Primary: Anti-MYLPF (SL5159R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 19 kD
Tissue/cell: muscle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Fast skeletal Myosin Polyclonal Antibody, Unconjugated(SL5159R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYLPF) Polyclonal Antibody, Unconjugated (SL5159R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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