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Product Name GOLPH2 Chinese Name 高尔基体膜蛋白GP73抗体 Alias Chromosome 9 open reading frame 155; Golgi membrane protein 1; Golgi membrane protein GP73; Golgi phosphoprotein 2; Golgi protein 73 kD; golgi protein 73kD; GOLM 1; GOLM1; GOLPH 2; GOLPH2; GP 73; GP73; PSEC0257; bA379P1.3; C9orf155. literatures Specific References (1) | SL4887R has been referenced in 1 publications.[IF=6.023] Ling Xie. et al. Suppression of GOLM1 by EGCG through HGF/HGFR/AKT/GSK-3β/β-catenin/c-Myc signaling pathway inhibits cell migration of MDA-MB-231. Food Chem Toxicol. 2021 Nov;157:112574 WB ; human.Research Area Tumour Signal transduction Bacteria and viruses Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 45kDa Cellular localization cytoplasmic The cell membrane Extracellular matrix Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GOLPH2: 21-120/401 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail GOLPH2 is widely expressed. Expression levels are high in the colon, prostate, trachea and stomach; expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. GOLPH2 is predominantly expressed by cells of the epithelial lineage. It is expressed at a low level in normal liver, though expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non viral causes (alcohol induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte derived syncitial giant cells. GOLPH2 is constitutively expressed by biliary epithelial cells but not by hepatocytes.
Function:
Unknown. Cellular response protein to viral infection.
Subcellular Location:
Golgi apparatus, cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.
Tissue Specificity:
Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte-derived syncitial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes.
Post-translational modifications:
Glycosylated.
Phosphorylation sites are present in the extracelllular medium.
Similarity:
Belongs to the GOLM1/CASC4 family.
SWISS:
Q8NBJ4
Gene ID:
51280
Database links:Entrez Gene: 51280 Human
Entrez Gene: 105348 Mouse
Omim: 606804 Human
SwissProt: Q8NBJ4 Human
SwissProt: Q91XA2 Mouse
Unigene: 494337 Human
Unigene: 171335 Mouse
Product Picture Sample:
A549(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti- TBX1 (SL4887R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
(human/cell line)A431 lysates probed with GOLPH2 Polyclonal Antibody, Unconjugated (Catalog #SL4887R) at 1:300 overnight at 4ⅹC. Followed by a conjugated secondary antibody (Secondary Catalog #926-32211) at 1:10000 for 60 min at 37ⅹC.Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (GOLPH2) Polyclonal/MonoclonalAntibody, Unconjugated (SL4887R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Paraformaldehyde-fixed, paraffin embedded (human stomach tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (GOLPH2) Polyclonal/MonoclonalAntibody, Unconjugated (SL4887R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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