Product Name	AFP
Chinese Name	甲胎蛋白抗体
Alias	Alpha Fetoprotein; Alpha-fetoprotein; AFP; alpha 1 fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.
literatures	Specific References  (4)     |     SL4708R has been referenced in 4 publications. [IF=6.914] Chang N et al. Low cost 3D microfluidic chips for multiplex protein detection based on photonic crystal beads. Lab Chip. 2018 Dec 7;18(23):3638-3644.  3D microfluidic chips ;  Human.   PubMed:30357200 [IF=6.408] Chellachamy Anbalagan, Amarnath. et al. Redox-labelled detection probe enabled immunoassay for simultaneous detection of multiple cancer biomarkers. MICROCHIM ACTA. 2023 Mar;190(3):1-9   PubMed:36757491 [IF=4.019] Li Y et al. Metal Coordination Polymer Induced Perylene Probe Excimer Fluorescence and Its Application in Acetylcholinesterase Sensing and Alpha-Fetoprotein Immunoassay. Analyst. 2019 Mar 21;144(6):2034-2041.  ELISA ;   PubMed:30702092 [IF=3.594] T H et al. Layer-by-layer self-assembly of MoS2/PDDA hybrid film in microfluidic chips for ultrasensitive electrochemical immunosensing of alpha-fetoprotein. Microchemical Journal,(2020) 158, 105209.  Other ;   PubMed:doi:10.1016/j.microc.2020.105209
Research Area	Tumour  Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Monkey, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	65kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human AFP: 521-609/609
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries. Function: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Subunit: Dimeric and trimeric forms have been found in addition to the monomeric form. Subcellular Location: Secreted. Tissue Specificity: Plasma. Synthesized by the fetal liver and yolk sac. Post-translational modifications: Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated. Similarity: Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains. SWISS: P02771 Gene ID: 174 Database links: Entrez Gene: 174 Human Omim: 104150 Human SwissProt: P02771 Human Unigene: 518808 Human AFP是由胚胎卵黄囊细胞，胚胎肝细胞和胎儿肠道细胞合成的一种glycoprotein。主要用于原发性或转移性肝细胞癌、肝样胃癌和性腺或性腺外某些生殖细胞Tumour(如内胚窦瘤)的诊断和鉴别诊断。 AFP敏感度较低，特异性强，大约60%左右的肝细胞肝癌阳性，在低分化Tumour中常呈阳性表达。
Product Picture	Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-AFP (SL4708R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD Sample: Hcclm3(Human) Cell Lysate at 30 ug Primary: Anti-AFP (SL4708R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AFP) Polyclonal Antibody, Unconjugated (SL4708R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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