Product Name	phospho-ROCK1 (Thr455+Ser456)
Chinese Name	磷酸化Rho相关蛋白激酶1抗体
Alias	p-ROCK1(Thr455/Ser456); p160 ROCK1; p160ROCK; Renal carcinoma antigen NY REN 35; Rho associated coiled coil containing protein kinase 1; Rho associated protein kinase 1; Rho-associated coiled-coil containing protein kinase 1; ROCK1_HUMAN.
literatures	Specific References  (7)     |     SL4630R has been referenced in 7 publications. [IF=6.162] Zhou Y et al. ROCK2 Confers Acquired Gemcitabine Resistance in Pancreatic Cancer Cells by Upregulating Transcription Factor ZEB1. Cancers (Basel). 2019 Nov 27;11(12). pii: E1881.  WB ;  Human.   PubMed:31783584 [IF=4.566] Xu Jiahui. et al. Temperature and Growth Selection Effects on Proliferation, Differentiation, and Adipogenic Potential of Turkey Myogenic Satellite Cells Through Frizzled-7-Mediated Wnt Planar Cell Polarity Pathway. FRONT PHYSIOL. 2022 May;0:889  WB ;  Chicken.   PubMed:35677087 [IF=4.15] Wang, Nan, et al. "Vascular endothelial growth factor stimulates endothelial differentiation from mesenchymal stem cells via Rho/myocardin-related transcription factor-A signaling pathway." The International Journal of Biochemistry & Cell Biology (2013).  WB ;  Rat.   PubMed:23624342 [IF=3.388] Kusuyama J et al. Low intensity pulsed ultrasound (LIPUS) maintains osteogenic potency by the increasedexpression and stability of Nanog through spleen tyrosine kinase (Syk) activation. Cell Signal. 2019 Jun 19;62:109345.  WB ;  Mouse.   PubMed:31228531 [IF=2.782] Choi et al. GM-CSF reduces expression of chondroitin sulfate proteoglycan (CSPG) core proteins in TGF-β-treated primary astrocytes. (2014) BMB.Re. 47:679-84  WB ;  Rat.   PubMed:24602609 [IF=1.74] Lin, Ping-Yuan, et al. "RhoA/ROCK1 regulates Avian Reovirus S1133-induced switch from autophagy to apoptosis." BMC veterinary research 11.1 (2015): 103.  WB ;   PubMed:25944062 [IF=0] Kim et al. Up-regulation of neogenin-1 increases cell proliferation and motility in gastric cancer. (2014) Oncotarget. 5:3386-98  WB ;  Human.   PubMed:24930499
Product Type	Phosphorylated anti
Research Area	Tumour  Signal transduction  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	158kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human ROCK1 around the phosphorylation site of Thr455+Ser456: CR(p-T)(p-S)N
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]. Function: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Subunit: Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton, centrosome, centriole (By similarity). Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Note=Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes. Tissue Specificity: Detected in blood platelets. Post-translational modifications: Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR. Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 REM (Hr1) repeat. SWISS: Q13464 Gene ID: 6093 Database links: Entrez Gene: 6093 Human Entrez Gene: 19877 Mouse Entrez Gene: 81762 Rat Omim: 601702 Human SwissProt: Q8MIT6 Cow SwissProt: Q13464 Human SwissProt: P70335 Mouse SwissProt: Q63644 Rat ROCK1也是丝/苏氨基酸激酶的一种，可调节cell factor，促进平滑肌收缩和肌动蛋白的形成,因此又称：含有卷曲螺旋的蛋白激酶。目前主要用于Tumour浸润、转移方面的研究。
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-phospho-ROCK1(Thr455+Ser456) (SL4630R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 158 kD Observed band size: 158 kD Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-ROCK1(Thr455/Ser456) Polyclonal Antibody, Unconjugated(SL4630R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ROCK1 (Thr455+Ser456)) Polyclonal Antibody, Unconjugated (SL4630R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-ROCK1 (Thr455+Ser456)) polyclonal Antibody, Unconjugated (SL4630R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control:MCF-7. Primary Antibody (green line): Rabbit Anti-phospho-ROCK1 (Thr455+Ser456) antibody (SL4630R) Dilution: 1ug/Test; Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）：Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:MCF-7. Primary Antibody (green line): Rabbit Anti-phospho-ROCK1 (Thr455+Ser456) antibody (SL4630R) Dilution: 1ug/Test; Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）：Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control(blue): Hela (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- phospho-ROCK1(Thr455+Ser456) antibody(SL4630R), Dilution:1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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