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Product Name PTPN20 Chinese Name 蛋白酪氨酸磷酸酶非受体型20抗体 Alias bA42B19.1; hPTPN20; Protein tyrosine phosphatase non receptor type 20; PTN20_HUMAN; PTP Typ; PTP Typ; Ptpn20; PTPN20A; PTPN20B; Tyrosine-protein phosphatase non-receptor type 20. Research Area Cell biology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Pig, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 48kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PTPN20: 261-360/420 Lsotype IgG Purification affinity purified by Protein A Buffer Solution Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The product of this gene belongs to the family of classical tyrosine-specific protein tyrosine phosphatases. Many protein tyrosine phosphatases have been shown to regulate fundamental cellular processes. The encoded protein appears to be targeted to sites of actin polymerization. A pseudogene of this gene has been defined on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
Function:
Tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. Has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates.
Subcellular Location:
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Colocalizes with the microtubule-organizing center and intracellular membrane compartments.
Tissue Specificity:
Present in many cell lines (at protein level). Widely expressed.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Belongs to the protein-tyrosine phosphatase family.
Non-receptor class subfamily.
Contains 1 tyrosine-protein phosphatase domain.
SWISS:
Q4JDL3
Gene ID:
26095
Database links:Entrez Gene: 26095 Human
Entrez Gene: 653129 Human
Omim: 610630 Human
Omim: 610631 Human
SwissProt: Q4JDL3 Human
Unigene: 440733 Human
Unigene: 499552 Human
Product Picture Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-PTPN20 (SL4431R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PTPN20) Polyclonal Antibody, Unconjugated (SL4431R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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