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Product Name PKN Chinese Name 蛋白激酶C相关激酶1抗体 Alias DBK; DPKN; PAK 1; PRK 1; PROTEIN KINASE C RELATED KINASE 1; SERINE/THREONINE PROTEIN KINASE N; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA. Research Area Tumour Cell biology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Sheep, ) Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 104kDa Cellular localization The nucleus cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PKN: 401-500/942 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Function:
Pkc-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. May play a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion and transcription activation signaling processes (By similarity). Plays a role in regulating Rho-mediated dorsal closure during embryogenesis.
Subunit:
Interacts (via N-terminus) with Rho1 (via REM repeats), Rac1 (via REM 1 repeat) and Rac2.
Subcellular Location:
Cytoplasmic.
Post-translational modifications:
Phosphorylated (By similarity). Autophosphorylated; autophosphorylation is stimulated by GTP-bound Rho/Rac GTPases.
Similarity:
Belongs to the protein kinase superfamily.
Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 1 protein kinase domain.
Contains 3 REM (Hr1) repeats.
SWISS:
Q16512
Gene ID:
5585
Database links:Entrez Gene: 5585 Human
Entrez Gene: 320795 Mouse
SwissProt: Q16512 Human
SwissProt: P70268 Mouse
Unigene: 466044 Human
Unigene: 213000 Mouse
Unigene: 49880 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PKN) Polyclonal Antibody, Unconjugated (SL4320R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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