Product Name	IL-6R alpha
Chinese Name	白细胞介素6受体α/IL-6Rα抗体
Alias	IL6R; CD 126; CD126; CD126 antigen; gp80; IL 6R 1; IL 6R alpha; IL 6R; IL-6 receptor subunit alpha; IL-6R 1; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; Il6r; IL6RA; IL6RA_HUMAN; Interleukin 6 receptor alpha chain; Interleukin 6 receptor; Interleukin-6 receptor subunit alpha; Membrane glycoprotein 80;
Research Area	Cell biology  immunology  The cell membrane受体  Cell Surface Molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:400-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	50kDa
Cellular localization	The cell membrane Secretory protein
Form	Liquid
immunogen	Recombinant human IL-6R alpha: 20-365/468
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M PBS(pH7.4)
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]. Function: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Subunit: Hexamer of two molecules each of IL6, IL6R and IL6ST. Subcellular Location: Isoform 1: Basolateral cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Tissue Specificity: Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum. Post-translational modifications: A short soluble form may also be released from the membrane by proteolysis. Similarity: Belongs to the type I cytokine receptor family. Type 3subfamily. Contains 1 fibronectin type-III domain. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. SWISS: P08887 Gene ID: 3570 Database links: Entrez Gene: 3570 Human Entrez Gene: 16194 Mouse SwissProt: P08887 Human SwissProt: P22272 Mouse SwissProt: P22273 Rat Unigene: 2856 Mouse
Product Picture	Sample: Lane 1:Recombinant Human IL-6R alpha Protein at 50ng Lane 1:Recombinant Human IL-6R alpha Protein at 500ng Primary: Rabbit Anti-IL-6R alpha Monoclonal Antibody at 1/1000 dilution (Cat.SLM-43015R) Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42kD Observed band size: 58-75kD Sample: Lane 1: Human IL-6R alpha Protein at 50ng Lane 2: Human IL-6R alpha Protein at 500ng Primary: Rabbit Anti-IL-6R alpha Protein Antibody at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42kD Observed band size: kD Sample: Lane 1: IL-6R alpha fusion protein (SL43015P) 0.2ug Lysates Primary: Anti-IL-6R alpha (SL43015R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50kDa Observed band size: 60kDa Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-6R alpha) Polyclonal Antibody, Unconjugated (SL43015R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-6R alpha) Polyclonal Antibody, Unconjugated (SL43015R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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