Product Name	DDIT4
Chinese Name	DNA损伤诱导转录蛋白4抗体
Alias	DDIT4; DDIT4_HUMAN; Dig2; DKFZp564O2071; DNA damage inducible transcript 4; DNA damage inducible transcript 4 protein; DNA damage-inducible transcript 4 protein; FLJ20500; HIF 1 responsive protein RTP801; HIF 1 responsive RTP801; HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD 1; REDD-1; REDD1; RP11 442H21.1; RTP801.
Research Area	Tumour  Cardiovascular  immunology  Chromatin and nuclear signals  The new supersedes the old  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Pig, Cow, Horse, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	25kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DDIT4: 165-232/232
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Function: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Subcellular Location: Cytoplasm. Tissue Specificity: Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level). Similarity: Belongs to the DDIT4 family. SWISS: Q9NX09 Gene ID: 54541 Database links: Entrez Gene: 54541 Human Omim: 607729 Human SwissProt: Q9NX09 Human Unigene: 523012 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DDIT4) Polyclonal Antibody, Unconjugated (SL4240R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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